An Acid‐Activatable Fluorescence Probe for Imaging Osteocytic Bone Resorption Activity in Deep Bone Cavities

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Abstract: A rationally designed pH‐activatable fluorescent probe (pHocas‐RIS) has been used to measure localised pH levels in osteocytic lacunae in bone tissue. Conjugation of the moderate bone‐binding drug risedronate to a pH‐activatable BODIPY fluorophore enables the probe to penetrate osteocytic lacunae cavities that are embedded deep within the bone matrix. After injection of pHocas‐RIS, any osteocytic lacunae caused by bone‐resorbing osteocytes cause the probe to fluoresce in vivo, thus allowing imaging by intravital two‐photon excitation microscopy. This pH responsive probe enabled the visualization of the bone mineralizing activities of acid producing osteocytes in real time, thus allowing the study of their central role in remodeling the bone‐matrix in healthy and disease states.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
An Acid‐Activatable Fluorescence Probe for Imaging Osteocytic Bone Resorption Activity in Deep Bone Cavities ; volume:59 ; number:47 ; year:2020 ; pages:20996-21000 ; extent:5
Angewandte Chemie / International edition. International edition ; 59, Heft 47 (2020), 20996-21000 (gesamt 5)

Urheber
Hashimoto, Ryu
Minoshima, Masafumi
Kikuta, Junichi
Yari, Shinya
Bull, Steven D.
Ishii, Masaru
Kikuchi, Kazuya

DOI
10.1002/anie.202006388
URN
urn:nbn:de:101:1-2022061314190826635409
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:35 MESZ

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Beteiligte

  • Hashimoto, Ryu
  • Minoshima, Masafumi
  • Kikuta, Junichi
  • Yari, Shinya
  • Bull, Steven D.
  • Ishii, Masaru
  • Kikuchi, Kazuya

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