Improving Gene Delivery: Synergy between Alkyl Chain Length and Lipoic Acid for PDMAEMA Hydrophobic Copolymers

Abstract: In the field of gene delivery, hydrophobic cationic copolymers hold great promise. They exhibit improved performance by effectively protecting genetic material from serum interactions while facilitating interactions with cellular membranes. However, managing cytotoxicity remains a significant challenge, prompting an investigation into suitable hydrophobic components. A particularly encouraging approach involves integrating nutrient components, like lipoic acid, which is known for its antioxidant properties and diverse cellular benefits such as cellular metabolism and growth. In this study, a copolymer library comprising 2‐(dimethylamino) ethyl methacrylate (DMAEMA) and lipoic acid methacrylate (LAMA), combined with either n‐butyl methacrylate (nBMA), ethyl methacrylate (EMA), or methyl methacrylate (MMA), is synthesized. This enables to probe the impact of lipoic acid incorporation while simultaneously exploring the influence of pendant acyclic alkyl chain length. The inclusion of lipoic acid results in a notable boost in transfection efficiency  while maintaining low cytotoxicity. Interestingly, higher levels of transfection efficiency are achieved in the presence of nBMA, EMA, or MMA. However, a positive correlation between pendant acyclic alkyl chain length and cytotoxicity is observed. Consequently, P (DMAEMA‐co‐LAMA‐co‐MMA), emerges as a promising candidate. This is attributed to the optimal combination of low cytotoxic MMA and transfection‐boosting LAMA, highlighting the crucial synergy between LAMA and MMA.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
Improving Gene Delivery: Synergy between Alkyl Chain Length and Lipoic Acid for PDMAEMA Hydrophobic Copolymers ; day:02 ; month:02 ; year:2024 ; extent:7
Macromolecular rapid communications ; (02.02.2024) (gesamt 7)

Urheber
Mapfumo, Prosper
Reichel, Liên S.
Hoeppener, Stephanie
Träger, Anja

DOI
10.1002/marc.202300649
URN
urn:nbn:de:101:1-2024020314024235561298
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:20 MESZ

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