DNAzyme‐Catalyzed Site‐Specific N‐Acylation of DNA Oligonucleotide Nucleobases

Abstract: We used in vitro selection to identify DNAzymes that acylate the exocyclic nucleobase amines of cytidine, guanosine, and adenosine in DNA oligonucleotides. The acyl donor was the 2,3,5,6‐tetrafluorophenyl ester (TFPE) of a 5′‐carboxyl oligonucleotide. Yields are as high as >95 % in 6 h. Several of the N‐acylation DNAzymes are catalytically active with RNA rather than DNA oligonucleotide substrates, and eight of nine DNAzymes for modifying C are site‐specific (>95 %) for one particular substrate nucleotide. These findings expand the catalytic ability of DNA to include site‐specific N‐acylation of oligonucleotide nucleobases. Future efforts will investigate the DNA and RNA substrate sequence generality of DNAzymes for oligonucleotide nucleobase N‐acylation, toward a universal approach for site‐specific oligonucleotide modification.

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch

Bibliographic citation
DNAzyme‐Catalyzed Site‐Specific N‐Acylation of DNA Oligonucleotide Nucleobases ; day:11 ; month:01 ; year:2024 ; extent:7
Angewandte Chemie ; (11.01.2024) (gesamt 7)

Creator
Kennebeck, Morgan M.
Kaminsky, Caroline K.
Massa, Maria A.
Das, Prakriti K.
Boyd, Robert D.
Bishka, Michelle
Tricarico, J. Tomas
Silverman, Scott K.

DOI
10.1002/ange.202317565
URN
urn:nbn:de:101:1-2024011214111966967059
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
15.08.2025, 7:24 AM CEST

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Associated

  • Kennebeck, Morgan M.
  • Kaminsky, Caroline K.
  • Massa, Maria A.
  • Das, Prakriti K.
  • Boyd, Robert D.
  • Bishka, Michelle
  • Tricarico, J. Tomas
  • Silverman, Scott K.

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