DNAzyme‐Catalyzed Site‐Specific N‐Acylation of DNA Oligonucleotide Nucleobases
Abstract: We used in vitro selection to identify DNAzymes that acylate the exocyclic nucleobase amines of cytidine, guanosine, and adenosine in DNA oligonucleotides. The acyl donor was the 2,3,5,6‐tetrafluorophenyl ester (TFPE) of a 5′‐carboxyl oligonucleotide. Yields are as high as >95 % in 6 h. Several of the N‐acylation DNAzymes are catalytically active with RNA rather than DNA oligonucleotide substrates, and eight of nine DNAzymes for modifying C are site‐specific (>95 %) for one particular substrate nucleotide. These findings expand the catalytic ability of DNA to include site‐specific N‐acylation of oligonucleotide nucleobases. Future efforts will investigate the DNA and RNA substrate sequence generality of DNAzymes for oligonucleotide nucleobase N‐acylation, toward a universal approach for site‐specific oligonucleotide modification.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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DNAzyme‐Catalyzed Site‐Specific N‐Acylation of DNA Oligonucleotide Nucleobases ; day:11 ; month:01 ; year:2024 ; extent:7
Angewandte Chemie ; (11.01.2024) (gesamt 7)
- Urheber
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Kennebeck, Morgan M.
Kaminsky, Caroline K.
Massa, Maria A.
Das, Prakriti K.
Boyd, Robert D.
Bishka, Michelle
Tricarico, J. Tomas
Silverman, Scott K.
- DOI
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10.1002/ange.202317565
- URN
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urn:nbn:de:101:1-2024011214111966967059
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:24 MESZ
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Beteiligte
- Kennebeck, Morgan M.
- Kaminsky, Caroline K.
- Massa, Maria A.
- Das, Prakriti K.
- Boyd, Robert D.
- Bishka, Michelle
- Tricarico, J. Tomas
- Silverman, Scott K.
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