Association of [alpha]/[beta]-hydrolase D16B with bovine conception rate and sperm plasma membrane lipid composition

Abstract: We have identified a Holstein sire named Tarantino who had been approved for artificial insemination that is based on normal semen characteristics (i.e., morphology, thermoresistance, motility, sperm concentration), but had no progeny after 412 first inseminations, resulting in a non-return rate (NRdev) of −29. Using whole genome association analysis and next generation sequencing, an associated nonsense variant in the α/β-hydrolase domain-containing 16B gene (ABHD16B) on bovine chromosome 13 was identified. The frequency of the mutant allele in the German Holstein population was determined to be 0.0018 in 222,645 investigated cattle specimens. The mutant allele was traced back to Whirlhill Kingpin (bornFeb. 13th, 1959) as potential founder. The expression of ABHD16B was detected by Western blotting and immunohistochemistry in testis and epididymis of control bulls. A lipidome comparison of the plasma membrane of fresh semen from carriers and controls showed significant differences in the concentration of phosphatidylcholine (PC), diacylglycerol (DAG), ceramide (Cer), sphingomyelin (SM), and phosphatidylcholine (-ether) (PC O-), indicating that ABHD16B plays a role in lipid biosynthesis. The altered lipid contents may explain the reduced fertilization ability of mutated sperms

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch
Anmerkungen
International journal of molecular sciences. - 21, 2 (2020) , 627, ISSN: 1422-0067

Schlagwort
Holstein
Sterilität

Ereignis
Veröffentlichung
(wo)
Freiburg
(wer)
Universität
(wann)
2020
Urheber
Shan, Shuwen
Xu, Fangzheng
Bleyer, Martina
Becker, Svenja
Melbaum, Torben
Wemheuer, Wilhelm
Hirschfeld, Marc
Wacker, Christin
Zhao, Shuhong
Schütz, Ekkehard
Brenig, Bertram

DOI
10.3390/ijms21020627
URN
urn:nbn:de:bsz:25-freidok-1553547
Rechteinformation
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Letzte Aktualisierung
25.03.2025, 13:50 MEZ

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Beteiligte

  • Shan, Shuwen
  • Xu, Fangzheng
  • Bleyer, Martina
  • Becker, Svenja
  • Melbaum, Torben
  • Wemheuer, Wilhelm
  • Hirschfeld, Marc
  • Wacker, Christin
  • Zhao, Shuhong
  • Schütz, Ekkehard
  • Brenig, Bertram
  • Universität

Entstanden

  • 2020

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