Membrane palmitoylated protein 2 is a synaptic scaffold protein required for synaptic SK2-containing channel function
Abstract: Mouse CA1 pyramidal neurons express apamin-sensitive SK2-containing channels in the post-synaptic membrane, positioned close to NMDA-type (N-methyl-D-aspartate) glutamate receptors. Activated by synaptically evoked NMDAR-dependent Ca2+ influx, the synaptic SK2- containing channels modulate excitatory post-synaptic responses and the induction of synaptic plasticity. In addition, their activity- and protein kinase A-dependent trafficking contributes to expression of long-term potentiation (LTP). We have identified a novel synaptic scaffold, MPP2 (membrane palmitoylated protein 2; p55), a member of the membrane-associated guanylate kinase (MAGUK) family that interacts with SK2-containing channels. MPP2 and SK2 co-immunopurified from mouse brain, and co-immunoprecipitated when they were co-expressed in HEK293 cells. MPP2 is highly expressed in the post-synaptic density of dendritic spines on CA1 pyramidal neurons. Knocking down MPP2 expression selectively abolished the SK2-containing channel contribution to synaptic responses and decreased LTP. Thus, MPP2 is a novel synaptic scaffold that is required for proper synaptic localization and function of SK2-containing channels
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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eLife. - 5 (2016) , e12637, ISSN: 2050-084X
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2019
- Urheber
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Kim, Gukhan
Luján, Rafael
Schwenk, Jochen
Kelley, Melissa H.
Aguado, Carolina
Watanabe, Masahiko
Fakler, Bernd
Maylie, James
Adelman, John P
- Beteiligte Personen und Organisationen
- DOI
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10.7554/elife.12637
- URN
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urn:nbn:de:bsz:25-freidok-1301637
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:58 MEZ
Datenpartner
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Beteiligte
- Kim, Gukhan
- Luján, Rafael
- Schwenk, Jochen
- Kelley, Melissa H.
- Aguado, Carolina
- Watanabe, Masahiko
- Fakler, Bernd
- Maylie, James
- Adelman, John P
- Albert-Ludwigs-Universität Freiburg. Physiologisches Institut
- Universität
Entstanden
- 2019
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