Label‐Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging
Abstract: Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label‐free in vivo imaging in small animals via side‐viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label‐free endomicroscopy for longitudinal studies for the first time.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Label‐Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging ; volume:6 ; number:8 ; year:2019 ; extent:8
Advanced science ; 6, Heft 8 (2019) (gesamt 8)
- Creator
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Dilipkumar, Ashwathama
Al‐Shemmary, Alaa
Kreiß, Lucas
Cvecek, Kristian
Carlé, Birgitta
Knieling, Ferdinand
Gonzales Menezes, Jean
Thoma, Oana‐Maria
Schmidt, Michael
Neurath, Markus F.
Waldner, Maximilian
Friedrich, Oliver
Schürmann, Sebastian
- DOI
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10.1002/advs.201801735
- URN
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urn:nbn:de:101:1-2022072706534709799134
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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17.04.2025, 3:31 PM CEST
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Associated
- Dilipkumar, Ashwathama
- Al‐Shemmary, Alaa
- Kreiß, Lucas
- Cvecek, Kristian
- Carlé, Birgitta
- Knieling, Ferdinand
- Gonzales Menezes, Jean
- Thoma, Oana‐Maria
- Schmidt, Michael
- Neurath, Markus F.
- Waldner, Maximilian
- Friedrich, Oliver
- Schürmann, Sebastian
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