A second gamma-glutamylpolyamine synthetase, GlnA2, is involved in polyamine catabolism in streptomyces coelicolor
Abstract: Streptomyces coelicolor is a soil bacterium living in a habitat with very changeable nutrient availability. This organism possesses a complex nitrogen metabolism and is able to utilize the polyamines putrescine, cadaverine, spermidine, and spermine and the monoamine ethanolamine. We demonstrated that GlnA2 (SCO2241) facilitates S. coelicolor to survive under high toxic polyamine concentrations. GlnA2 is a gamma-glutamylpolyamine synthetase, an enzyme catalyzing the first step in polyamine catabolism. The role of GlnA2 was confirmed in phenotypical studies with a glnA2 deletion mutant as well as in transcriptional and biochemical analyses. Among all GS-like enzymes in S. coelicolor, GlnA2 possesses the highest specificity towards short-chain polyamines (putrescine and cadaverine), while its functional homolog GlnA3 (SCO6962) prefers long-chain polyamines (spermidine and spermine) and GlnA4 (SCO1613) accepts only monoamines. The genome-wide RNAseq analysis in the presence of the polyamines putrescine, cadaverine, spermidine, or spermine revealed indication of the occurrence of different routes for polyamine catabolism in S. coelicolor involving GlnA2 and GlnA3. Furthermore, GlnA2 and GlnA3 are differently regulated. From our results, we can propose a complemented model of polyamine catabolism in S. coelicolor, which involves the gamma-glutamylation pathway as well as other alternative utilization pathways
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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International journal of molecular sciences. - 23, 7 (2022) , 3752, ISSN: 1422-0067
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2022
- Urheber
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Krysenko, Sergii
Okoniewski, Nicole
Nentwich, Merle
Matthews, Arne
Bäuerle, Moritz
Zinser, Alina
Busche, Tobias
Kulik, Andreas
Gursch, Stephanie
Kemeny, Annika
Bera, Agnieszka
Wohlleben, Wolfgang
- DOI
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10.3390/ijms23073752
- URN
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urn:nbn:de:bsz:25-freidok-2300695
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:24 MESZ
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Beteiligte
- Krysenko, Sergii
- Okoniewski, Nicole
- Nentwich, Merle
- Matthews, Arne
- Bäuerle, Moritz
- Zinser, Alina
- Busche, Tobias
- Kulik, Andreas
- Gursch, Stephanie
- Kemeny, Annika
- Bera, Agnieszka
- Wohlleben, Wolfgang
- Universität
Entstanden
- 2022
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