A second gamma-glutamylpolyamine synthetase, GlnA2, is involved in polyamine catabolism in streptomyces coelicolor
Abstract: Streptomyces coelicolor is a soil bacterium living in a habitat with very changeable nutrient availability. This organism possesses a complex nitrogen metabolism and is able to utilize the polyamines putrescine, cadaverine, spermidine, and spermine and the monoamine ethanolamine. We demonstrated that GlnA2 (SCO2241) facilitates S. coelicolor to survive under high toxic polyamine concentrations. GlnA2 is a gamma-glutamylpolyamine synthetase, an enzyme catalyzing the first step in polyamine catabolism. The role of GlnA2 was confirmed in phenotypical studies with a glnA2 deletion mutant as well as in transcriptional and biochemical analyses. Among all GS-like enzymes in S. coelicolor, GlnA2 possesses the highest specificity towards short-chain polyamines (putrescine and cadaverine), while its functional homolog GlnA3 (SCO6962) prefers long-chain polyamines (spermidine and spermine) and GlnA4 (SCO1613) accepts only monoamines. The genome-wide RNAseq analysis in the presence of the polyamines putrescine, cadaverine, spermidine, or spermine revealed indication of the occurrence of different routes for polyamine catabolism in S. coelicolor involving GlnA2 and GlnA3. Furthermore, GlnA2 and GlnA3 are differently regulated. From our results, we can propose a complemented model of polyamine catabolism in S. coelicolor, which involves the gamma-glutamylation pathway as well as other alternative utilization pathways
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Notes
-
International journal of molecular sciences. - 23, 7 (2022) , 3752, ISSN: 1422-0067
- Event
-
Veröffentlichung
- (where)
-
Freiburg
- (who)
-
Universität
- (when)
-
2022
- Creator
-
Krysenko, Sergii
Okoniewski, Nicole
Nentwich, Merle
Matthews, Arne
Bäuerle, Moritz
Zinser, Alina
Busche, Tobias
Kulik, Andreas
Gursch, Stephanie
Kemeny, Annika
Bera, Agnieszka
Wohlleben, Wolfgang
- DOI
-
10.3390/ijms23073752
- URN
-
urn:nbn:de:bsz:25-freidok-2300695
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:24 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Krysenko, Sergii
- Okoniewski, Nicole
- Nentwich, Merle
- Matthews, Arne
- Bäuerle, Moritz
- Zinser, Alina
- Busche, Tobias
- Kulik, Andreas
- Gursch, Stephanie
- Kemeny, Annika
- Bera, Agnieszka
- Wohlleben, Wolfgang
- Universität
Time of origin
- 2022
Other Objects (12)
Polyamine catabolism adds fuel to leaf senescence
Characterization of polyamine and ethanolamine utilization pathways in Streptomyces coelicolor M145
M. mazei glutamine synthetase and glutamine synthetase-GlnK1 structures reveal enzyme regulation by oligomer modulation
GmPAO-mediated polyamine catabolism enhances soybean Phytophthora resistance without growth penalty
Regulation des Stickstoff-Metabolismus von Streptomyces coelicolor M145 : Charakterisierung des globalen Transkriptionsfaktors GlnR
Polynomial representations of GLn [GLn]
Syringaldazine Peroxidase Stimulates Lignification by Enhancing Polyamine Catabolism in Wheat during Heat and Drought Stress
Modulation of Antioxidant Defense System and Polyamine Catabolism in Rice Leaves under Two Planting Conditions
Die Funktion von GlnA2 in der transkriptionellen und posttranslationalen Kontrolle des Stickstoffmetabolismus in Streptomyces coelicolor M145
Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria
Büssing VI GLn
II. Streptothrix coelicolor.
Polyamine catabolism adds fuel to leaf senescence
Characterization of polyamine and ethanolamine utilization pathways in Streptomyces coelicolor M145
M. mazei glutamine synthetase and glutamine synthetase-GlnK1 structures reveal enzyme regulation by oligomer modulation
GmPAO-mediated polyamine catabolism enhances soybean Phytophthora resistance without growth penalty
Regulation des Stickstoff-Metabolismus von Streptomyces coelicolor M145 : Charakterisierung des globalen Transkriptionsfaktors GlnR
Polynomial representations of GLn [GLn]
Syringaldazine Peroxidase Stimulates Lignification by Enhancing Polyamine Catabolism in Wheat during Heat and Drought Stress
Modulation of Antioxidant Defense System and Polyamine Catabolism in Rice Leaves under Two Planting Conditions
Die Funktion von GlnA2 in der transkriptionellen und posttranslationalen Kontrolle des Stickstoffmetabolismus in Streptomyces coelicolor M145
Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria
Büssing VI GLn
II. Streptothrix coelicolor.
Polyamine catabolism adds fuel to leaf senescence
Characterization of polyamine and ethanolamine utilization pathways in Streptomyces coelicolor M145
M. mazei glutamine synthetase and glutamine synthetase-GlnK1 structures reveal enzyme regulation by oligomer modulation
GmPAO-mediated polyamine catabolism enhances soybean Phytophthora resistance without growth penalty
Regulation des Stickstoff-Metabolismus von Streptomyces coelicolor M145 : Charakterisierung des globalen Transkriptionsfaktors GlnR
Polynomial representations of GLn [GLn]
Syringaldazine Peroxidase Stimulates Lignification by Enhancing Polyamine Catabolism in Wheat during Heat and Drought Stress
Modulation of Antioxidant Defense System and Polyamine Catabolism in Rice Leaves under Two Planting Conditions
Die Funktion von GlnA2 in der transkriptionellen und posttranslationalen Kontrolle des Stickstoffmetabolismus in Streptomyces coelicolor M145
Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria
Büssing VI GLn