Spatiotemporal regulation of FMNL2 by N-terminal myristoylation and C-terminal phosphorylation drives rapid filopodia formation
Abstract: The actin nucleating and polymerizing formin-like 2 (FMNL2) is upregulated in several cancers and has been shown to play important roles in cell migration, invasion, cell–cell adhesion and filopodia formation. Here, using structured illumination microscopy we show that FMNL2 promotes rapid and highly dynamic filopodia formation in epithelial cells while remaining on the tip of the growing filopodia. This filopodia tip localization depends fully on its N-terminal myristoylation. We further show that FMNL2-dependent filopodia formation requires its serine 1072 phosphorylation within the diaphanous-autoregulatory domain (DAD) by protein kinase C (PKC) α. Consistent with this, filopodia formation depends on PKC activity and PKCα localizes to the base of growing filopodia. Thus, a PKCα–FMNL2 signaling module spatiotemporally controls dynamic filopodia formation
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
Biomolecules. - 13, 3 (2023) , 548, ISSN: 2218-273X
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2023
- Urheber
- DOI
-
10.3390/biom13030548
- URN
-
urn:nbn:de:bsz:25-freidok-2352803
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
25.03.2025, 13:50 MEZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Lorenzen, Lina
- Frank, Dennis
- Schwan, Carsten
- Grosse, Robert
- Universität
Entstanden
- 2023
Ähnliche Objekte (12)
Structure-activity relationship studies of albicidin’s C-terminal dipeptide and N-terminal cinnamoyl residue
Differential phosphorylation of the N-terminal extension regulates phytochrome B signaling
The Host-Pathogen interaction of human cyclophilin A and HIV-1 Vpr requires specific N-terminal and novel C-terminal domains
Towards dissecting the mechanism of protein phosphatase‐1 inhibition by its c‐terminal phosphorylation
Einsatz des Tandem-Affinity-Purification-Verfahrens zum Nachweis von Keratin 23 Interaktionspartnern in Abhängigkeit vom N-terminal bzw C-terminal getaggten Zytokeratin 23
Cotranslational folding of HemK C-terminal domain
Role of differential phosphorylation of c-Jun N-terminal domain in degenerative and inflammatory pathways of CNS
Spatiotemporal calcium-dynamics in presynaptic terminals
Spatiotemporal calcium-dynamics in presynaptic terminals
NAC controls cotranslational N-terminal methionine excision in eukaryotes
Functional analysis of the N-terminal domain of SPA1
Comparison of the Clinical Performances of the Immunoenzymometric Assays for N-Terminal and C-Terminal Type I Collagen Telopeptides and the HPLC Assay for Pyridinium Cross-Links
Structure-activity relationship studies of albicidin’s C-terminal dipeptide and N-terminal cinnamoyl residue
Differential phosphorylation of the N-terminal extension regulates phytochrome B signaling
The Host-Pathogen interaction of human cyclophilin A and HIV-1 Vpr requires specific N-terminal and novel C-terminal domains
Towards dissecting the mechanism of protein phosphatase‐1 inhibition by its c‐terminal phosphorylation
Einsatz des Tandem-Affinity-Purification-Verfahrens zum Nachweis von Keratin 23 Interaktionspartnern in Abhängigkeit vom N-terminal bzw C-terminal getaggten Zytokeratin 23
Cotranslational folding of HemK C-terminal domain
Role of differential phosphorylation of c-Jun N-terminal domain in degenerative and inflammatory pathways of CNS
Spatiotemporal calcium-dynamics in presynaptic terminals
Spatiotemporal calcium-dynamics in presynaptic terminals
NAC controls cotranslational N-terminal methionine excision in eukaryotes
Functional analysis of the N-terminal domain of SPA1
Comparison of the Clinical Performances of the Immunoenzymometric Assays for N-Terminal and C-Terminal Type I Collagen Telopeptides and the HPLC Assay for Pyridinium Cross-Links
Structure-activity relationship studies of albicidin’s C-terminal dipeptide and N-terminal cinnamoyl residue
Differential phosphorylation of the N-terminal extension regulates phytochrome B signaling
The Host-Pathogen interaction of human cyclophilin A and HIV-1 Vpr requires specific N-terminal and novel C-terminal domains
Towards dissecting the mechanism of protein phosphatase‐1 inhibition by its c‐terminal phosphorylation
Einsatz des Tandem-Affinity-Purification-Verfahrens zum Nachweis von Keratin 23 Interaktionspartnern in Abhängigkeit vom N-terminal bzw C-terminal getaggten Zytokeratin 23
Cotranslational folding of HemK C-terminal domain
Role of differential phosphorylation of c-Jun N-terminal domain in degenerative and inflammatory pathways of CNS
Spatiotemporal calcium-dynamics in presynaptic terminals
Spatiotemporal calcium-dynamics in presynaptic terminals
NAC controls cotranslational N-terminal methionine excision in eukaryotes
Functional analysis of the N-terminal domain of SPA1