Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry
Abstract: Two heptapeptides have been prepared by Fmoc methodology using Wang resin as solid support. For attachment of the first amino acid, several coupling systems were evaluated, and DIC/DMAP system could give yields of >99% and low levels of racemization. The selection of scavenger combination to deprotect side chains revealed that H2O/p-cresol was good at scavenging trityl and 1,2-ethanedithiol was highly efficient for scavenging t-butyl. Through shortening the preactivation time to 5 min, the racemization which occurred during formation of amide bonds coupled by HBTU was minimized. The crude peptides were characterized by RP-HPLC and MS, and sequenced by MS/MS to acquire reliable amino acid sequence information.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry ; volume:4 ; number:2 ; year:2006 ; pages:285-298 ; extent:14
Open chemistry ; 4, Heft 2 (2006), 285-298 (gesamt 14)
- Urheber
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Jia, Chenxi
Qi, Wei
He, Zhimin
Yang, Haoming
Qiao, Bin
- DOI
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10.2478/s11532-006-0004-6
- URN
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urn:nbn:de:101:1-2410181541063.263159947655
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:35 MESZ
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Beteiligte
- Jia, Chenxi
- Qi, Wei
- He, Zhimin
- Yang, Haoming
- Qiao, Bin
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