Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry
Abstract: Two heptapeptides have been prepared by Fmoc methodology using Wang resin as solid support. For attachment of the first amino acid, several coupling systems were evaluated, and DIC/DMAP system could give yields of >99% and low levels of racemization. The selection of scavenger combination to deprotect side chains revealed that H2O/p-cresol was good at scavenging trityl and 1,2-ethanedithiol was highly efficient for scavenging t-butyl. Through shortening the preactivation time to 5 min, the racemization which occurred during formation of amide bonds coupled by HBTU was minimized. The crude peptides were characterized by RP-HPLC and MS, and sequenced by MS/MS to acquire reliable amino acid sequence information.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry ; volume:4 ; number:2 ; year:2006 ; pages:285-298 ; extent:14
Open chemistry ; 4, Heft 2 (2006), 285-298 (gesamt 14)
- Creator
-
Jia, Chenxi
Qi, Wei
He, Zhimin
Yang, Haoming
Qiao, Bin
- DOI
-
10.2478/s11532-006-0004-6
- URN
-
urn:nbn:de:101:1-2410181541063.263159947655
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:35 AM CEST
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Jia, Chenxi
- Qi, Wei
- He, Zhimin
- Yang, Haoming
- Qiao, Bin
Other Objects (12)
A novel approach to denoising ion trap tandem mass spectra
Ion mobility-tandem mass spectrometry of mucin-type O-glycans
Case series: TRAP sequence
Ion mobility spectrometry-tandem mass spectrometry strategies for the on-line monitoring of a continuous microflow reaction
Mass spectrometry, mass spectrometry : techniques and applications of tandem mass spectrometry
Surface-Electrode Ion Trap Development
Progressive search in tandem mass spectrometry
Two-dimensional mass spectrometry: new perspectives for tandem mass spectrometry
Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry
Sequencing peptides by electrospray ion-trap mass spectrometry: A useful tool in synthesis of Axinastatin 3
Highly Efficient Ion Manipulator for Tandem Ion Mobility Spectrometry: Exploring a Versatile Technique by a Study of Primary Alcohols
Rapid Screening of Ellagitannins in Natural Sources via Targeted Reporter Ion Triggered Tandem Mass Spectrometry
A novel approach to denoising ion trap tandem mass spectra
Ion mobility-tandem mass spectrometry of mucin-type O-glycans
Case series: TRAP sequence
Ion mobility spectrometry-tandem mass spectrometry strategies for the on-line monitoring of a continuous microflow reaction
Mass spectrometry, mass spectrometry : techniques and applications of tandem mass spectrometry
Surface-Electrode Ion Trap Development
Progressive search in tandem mass spectrometry
Two-dimensional mass spectrometry: new perspectives for tandem mass spectrometry
Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry
Sequencing peptides by electrospray ion-trap mass spectrometry: A useful tool in synthesis of Axinastatin 3
Highly Efficient Ion Manipulator for Tandem Ion Mobility Spectrometry: Exploring a Versatile Technique by a Study of Primary Alcohols
Rapid Screening of Ellagitannins in Natural Sources via Targeted Reporter Ion Triggered Tandem Mass Spectrometry
A novel approach to denoising ion trap tandem mass spectra
Ion mobility-tandem mass spectrometry of mucin-type O-glycans
Case series: TRAP sequence
Ion mobility spectrometry-tandem mass spectrometry strategies for the on-line monitoring of a continuous microflow reaction
Mass spectrometry, mass spectrometry : techniques and applications of tandem mass spectrometry
Surface-Electrode Ion Trap Development
Progressive search in tandem mass spectrometry
Two-dimensional mass spectrometry: new perspectives for tandem mass spectrometry
Determination of sulfation pattern in brain glycosaminoglycans by chip-based electrospray ionization ion trap mass spectrometry
Sequencing peptides by electrospray ion-trap mass spectrometry: A useful tool in synthesis of Axinastatin 3
Highly Efficient Ion Manipulator for Tandem Ion Mobility Spectrometry: Exploring a Versatile Technique by a Study of Primary Alcohols