The viability of cell that encapsulated in calcium alginate hydrogel beads
Objective: To prove that calcium alginate beads can be used as scaffolds during in vitro culture. Methods: Mouse preosteoblastic cells (MC3T3-E1) were encapsulated in calcium alginate hydrogel beads. The Cell Counting Kit-8 (CCK-8) assay was used to assess cell viability at 2, 5, 8, 11, 14, and 21 days. Calcein-AM and propidium iodide (PI) were employed for live/dead staining. Results: MC3T3-E1 cells were alive on day 21 and had the highest viability on day 14. Conclusion: MC3T3-E1 cells could be encapsulated in calcium alginate hydrogel beads and cultured. Calcium alginate hydrogel beads can be used as scaffolds for three-dimensional in vitro culture.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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The viability of cell that encapsulated in calcium alginate hydrogel beads ; volume:29 ; number:1 ; year:2022 ; pages:473-480 ; extent:8
Science and engineering of composite materials ; 29, Heft 1 (2022), 473-480 (gesamt 8)
- Creator
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Li, Fang-Fang
Tang, Wei-Feng
Xie, Qiu-Fei
- DOI
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10.1515/secm-2022-0156
- URN
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urn:nbn:de:101:1-2022120913024100590147
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:36 AM CEST
Data provider
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Li, Fang-Fang
- Tang, Wei-Feng
- Xie, Qiu-Fei
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