Pancreatic cancer: circulating tumor cells and primary tumors show heterogeneous KRAS mutations

Abstract: Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease. Circulating tumor cells (CTC) in the blood are hypothesized as the means of systemic tumor spread. Blood obtained from healthy donors and patients with PDAC was therefore subject to size-based CTC-isolation (ISET). We additionally compared Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations in pancreatic CTC and corresponding tumors, and evaluated their significance as prognostic markers. Samples from 68 individuals (58 PDAC patients, 10 healthy donors) were analyzed; CTCs were present in patients with UICC stage IA-IV tumors and none of the controls (p < 0.001). Patients with >3 CTC/ml had a trend for worse median overall survival (OS) than patients with 0.3–3 CTC/ml (P = 0.12). Surprisingly, CTCs harbored various KRAS mutations in codon 12 and 13. Patients with a KRASG12V mutation in their CTC (n = 14) had a trend to better median OS (24.5 months) compared to patients with other (10 months), or no detectable KRAS mutations (8 months; P = 0.04). KRAS mutations in CTC and corresponding tumor were discordant in 11 of 26 “tumor-CTC-pairs” (42%), while 15 (58%) had a matching mutation; survival was similar in both groups (P = 0.36). Genetic characterization, including mutations such as KRAS, may prove useful for prognosis and understanding of tumor biology

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: Scientific reports 7 (2017), 4510, DOI 10.1038/s41598-017-04601-z, issn: 2045-2322 ; IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Klassifikation und Themen

• Schlagwort: Krebs ; Bauchspeicheldrüsenkrebs ; Tumorzelle ; Onkologie

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2017
• Urheber: Kulemann, Birte ; Rösch, Stephanie ; Seifert, Sindy ; Timme-Bronsert, Sylvia ; Bronsert, Peter ; Seifert, Gabriel ; Martini, Verena ; Kuvendjiska, Jasmina ; Glatz, Torben ; Hussung, Saskia ; Fritsch, Ralph ; Becker, Heiko ; Höppner, Jens
• Beteiligte Personen und Organisationen: Universitätsklinikum Freiburg. Department Chirurgie ; Institut für Klinische Pathologie. Freiburg im Breisgau ; Tumorzentrum Freiburg - CCCF ; Universitätsklinikum Freiburg. Klinik für Innere Medizin I ; Albert-Ludwigs-Universität Freiburg. Medizinische Fakultät ; DKTK German Cancer Consortium ; Albert-Ludwigs-Universität Freiburg

Weitere Informationen

• DOI: 10.1038/s41598-017-04601-z
• URN: urn:nbn:de:bsz:25-freidok-128845
• Rechteinformation: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 25.03.2025, 13:51 MEZ