Blocking mitochondrial pyruvate import in brown adipocytes induces energy wasting via lipid cycling
Abstract: Combined fatty acid esterification and lipolysis, termed lipid cycling, is an ATP‐consuming process that contributes to energy expenditure. Therefore, interventions that stimulate energy expenditure through lipid cycling are of great interest. Here we find that pharmacological and genetic inhibition of the mitochondrial pyruvate carrier (MPC) in brown adipocytes activates lipid cycling and energy expenditure, even in the absence of adrenergic stimulation. We show that the resulting increase in ATP demand elevates mitochondrial respiration coupled to ATP synthesis and fueled by lipid oxidation. We identify that glutamine consumption and the Malate‐Aspartate Shuttle are required for the increase in Energy Expenditure induced by MPC inhibition in Brown Adipocytes (MAShEEBA). We thus demonstrate that energy expenditure through enhanced lipid cycling can be activated in brown adipocytes by decreasing mitochondrial pyruvate availability. We present a new mechanism to increase energy expenditure and fat oxidation in brown adipocytes, which does not require adrenergic stimulation of mitochondrial uncoupling.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Blocking mitochondrial pyruvate import in brown adipocytes induces energy wasting via lipid cycling ; volume:21 ; number:12 ; year:2020 ; extent:17
EMBO reports / European Molecular Biology Organization ; 21, Heft 12 (2020) (gesamt 17)
- Creator
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Veliova, Michaela
Ferreira, Caroline M.
Benador, Ilan Y.
Jones, Anthony E.
Mahdaviani, Kiana
Brownstein, Alexandra J.
Desousa, Brandon R.
Acín‐Pérez, Rebeca
Petcherski, Anton
Assali, Essam A.
Stiles, Linsey
Divakaruni, Ajit S.
Prentki, Marc
Corkey, Barbara E.
Liesa, Marc
Oliveira, Marcus F.
Shirihai, Orian S.
- DOI
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10.15252/embr.201949634
- URN
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urn:nbn:de:101:1-2022070407293756300420
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:28 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Veliova, Michaela
- Ferreira, Caroline M.
- Benador, Ilan Y.
- Jones, Anthony E.
- Mahdaviani, Kiana
- Brownstein, Alexandra J.
- Desousa, Brandon R.
- Acín‐Pérez, Rebeca
- Petcherski, Anton
- Assali, Essam A.
- Stiles, Linsey
- Divakaruni, Ajit S.
- Prentki, Marc
- Corkey, Barbara E.
- Liesa, Marc
- Oliveira, Marcus F.
- Shirihai, Orian S.
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Hypoxia Aggravates Inactivity-Related Muscle Wasting
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Blocking extracellular activation of myostatin as a strategy for treating muscle wasting
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Cerebral salt wasting syndrome
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Wasting in chronic kidney disease
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Hypoxia Aggravates Inactivity-Related Muscle Wasting
Cardiac wasting in patients with cancer