Flavonol‐mediated stabilization of PIN efflux complexes regulates polar auxin transport
Abstract: The transport of auxin controls the rate, direction and localization of plant growth and development. The course of auxin transport is defined by the polar subcellular localization of the PIN proteins, a family of auxin efflux transporters. However, little is known about the composition and regulation of the PIN protein complex. Here, using blue‐native PAGE and quantitative mass spectrometry, we identify native PIN core transport units as homo‐ and heteromers assembled from PIN1, PIN2, PIN3, PIN4 and PIN7 subunits only. Furthermore, we show that endogenous flavonols stabilize PIN dimers to regulate auxin efflux in the same way as does the auxin transport inhibitor 1‐naphthylphthalamic acid (NPA). This inhibitory mechanism is counteracted both by the natural auxin indole‐3‐acetic acid and by phosphomimetic amino acids introduced into the PIN1 cytoplasmic domain. Our results lend mechanistic insights into an endogenous control mechanism which regulates PIN function and opens the way for a deeper understanding of the protein environment and regulation of the polar auxin transport complex
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Edition
-
Online first OA
- Language
-
Englisch
- Notes
-
The EMBO journal. - 40, 1 (2021) , e104416, ISSN: 1460-2075
- Event
-
Veröffentlichung
- (where)
-
Freiburg
- (who)
-
Universität
- (when)
-
2020
- Creator
-
Teale, William
Pasternak, Taras
Dal Bosco, Cristina
Dovzhenko, Alexander
Kratzat, Krystyna
Bildl, Wolfgang
Schwörer, Manuel
Falk, Thorsten
Ruperti, Benedetto
Schaefer, Jonas V.
Shahriari, Mojgan
Pilgermayer, Lena
Li, Xugang
Lübben, Florian
Plückthun, Andreas
Schulte, Uwe
Palme, Klaus
- DOI
-
10.15252/embj.2020104416
- URN
-
urn:nbn:de:bsz:25-freidok-1697418
- Rights
-
Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
25.03.2025, 1:42 PM CET
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Teale, William
- Pasternak, Taras
- Dal Bosco, Cristina
- Dovzhenko, Alexander
- Kratzat, Krystyna
- Bildl, Wolfgang
- Schwörer, Manuel
- Falk, Thorsten
- Ruperti, Benedetto
- Schaefer, Jonas V.
- Shahriari, Mojgan
- Pilgermayer, Lena
- Li, Xugang
- Lübben, Florian
- Plückthun, Andreas
- Schulte, Uwe
- Palme, Klaus
- Universität
Time of origin
- 2020
Other Objects (12)
Biochemical and physiological characterization of PIN auxin efflux carriers from Arabidopsis thaliana
Auxin transport regulation through dynamic efflux carrier phosphorylation
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis
Auxin regulates SNARE-dependent vacuolar morphology restricting cell size
Auxin transport through PIN6 regulates drought tolerance in Arabidopsis
Plasma membrane t-SNARE AtSYP132 regulates intracellular trafficking of auxin efflux carrier PIN1 and root apical meristem maintenance in Arabidopsis thaliana
Regulation of PIN-FORMED-mediated auxin transport and the role of auxin and cytokinin responses in plant-nematode interactions
PIN-LIKES coordinate brassinosteroid signaling with nuclear auxin input in arabidopsis thaliana
Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane
Polar auxin transport
Auxin-induced plasma membrane depolarization is regulated by Auxin transport and not by Auxin binding protein1
Auxin signal in tobacco BY-2 cells - Actin sensory role for auxin and auxin subcellular distribution
Biochemical and physiological characterization of PIN auxin efflux carriers from Arabidopsis thaliana
Auxin transport regulation through dynamic efflux carrier phosphorylation
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis
Auxin regulates SNARE-dependent vacuolar morphology restricting cell size
Auxin transport through PIN6 regulates drought tolerance in Arabidopsis
Plasma membrane t-SNARE AtSYP132 regulates intracellular trafficking of auxin efflux carrier PIN1 and root apical meristem maintenance in Arabidopsis thaliana
Regulation of PIN-FORMED-mediated auxin transport and the role of auxin and cytokinin responses in plant-nematode interactions
PIN-LIKES coordinate brassinosteroid signaling with nuclear auxin input in arabidopsis thaliana
Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane
Polar auxin transport
Auxin-induced plasma membrane depolarization is regulated by Auxin transport and not by Auxin binding protein1
Auxin signal in tobacco BY-2 cells - Actin sensory role for auxin and auxin subcellular distribution
Biochemical and physiological characterization of PIN auxin efflux carriers from Arabidopsis thaliana
Auxin transport regulation through dynamic efflux carrier phosphorylation
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis
Auxin regulates SNARE-dependent vacuolar morphology restricting cell size
Auxin transport through PIN6 regulates drought tolerance in Arabidopsis
Plasma membrane t-SNARE AtSYP132 regulates intracellular trafficking of auxin efflux carrier PIN1 and root apical meristem maintenance in Arabidopsis thaliana
Regulation of PIN-FORMED-mediated auxin transport and the role of auxin and cytokinin responses in plant-nematode interactions
PIN-LIKES coordinate brassinosteroid signaling with nuclear auxin input in arabidopsis thaliana
Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane
Polar auxin transport
Auxin-induced plasma membrane depolarization is regulated by Auxin transport and not by Auxin binding protein1