Monitoring the Reversibility of GPCR Signaling by Combining Photochromic Ligands with Label‐free Impedance Analysis
Abstract: G protein‐coupled cell surface receptors (GPCR) trigger complex intracellular signaling cascades upon agonist binding. Classic pharmacological assays provide information about binding affinities, activation or blockade at different stages of the signaling cascade, but real time dynamics and reversibility of these processes remain often disguised. We show that combining photochromic NPY receptor ligands, which can be toggled in their receptor activation ability by irradiation with light of different wavelengths, with whole cell label‐free impedance assays allows observing the cell response to receptor activation and its reversibility over time. The concept demonstrated on NPY receptors may be well applicable to many other GPCRs providing a deeper insight into the time course of intracellular signaling processes.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Monitoring the Reversibility of GPCR Signaling by Combining Photochromic Ligands with Label‐free Impedance Analysis ; day:18 ; month:04 ; year:2023 ; extent:12
Angewandte Chemie / International edition. International edition ; (18.04.2023) (gesamt 12)
- Creator
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Wirth, Ulrike
Erl, Julia
Azzam, Saphia
Höring, Carina
Skiba, Michael
Singh, Ritu
Hochmuth, Kathrin
Keller, Max
Wegener, Joachim
König, Burkhard
- DOI
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10.1002/anie.202215547
- URN
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urn:nbn:de:101:1-2023041915055410010402
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:58 AM CEST
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Associated
- Wirth, Ulrike
- Erl, Julia
- Azzam, Saphia
- Höring, Carina
- Skiba, Michael
- Singh, Ritu
- Hochmuth, Kathrin
- Keller, Max
- Wegener, Joachim
- König, Burkhard
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Functionalized Photochromic Scaffolds
Synthese, Struktur und Eigenschaften reaktiver Makrocyclen und nicht-kovalenter Aggregate
Die Kristallstruktur von Kaliumtrichlorozinkat-Monohydrat KZnCl3 · 1 H2O
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Visible‐Light‐Mediated Photoredox‐Catalyzed N ‐Arylation of NH ‐Sulfoximines with Electron‐Rich Arenes
Conversion of carbohydrates in low melting mixtures and Melanoma Inhibitory Activity (MIA) protein inhibitors for the treatment of malignant melanoma
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