Monitoring the Reversibility of GPCR Signaling by Combining Photochromic Ligands with Label‐free Impedance Analysis
Abstract: G protein‐coupled cell surface receptors (GPCR) trigger complex intracellular signaling cascades upon agonist binding. Classic pharmacological assays provide information about binding affinities, activation or blockade at different stages of the signaling cascade, but real time dynamics and reversibility of these processes remain often disguised. We show that combining photochromic NPY receptor ligands, which can be toggled in their receptor activation ability by irradiation with light of different wavelengths, with whole cell label‐free impedance assays allows observing the cell response to receptor activation and its reversibility over time. The concept demonstrated on NPY receptors may be well applicable to many other GPCRs providing a deeper insight into the time course of intracellular signaling processes.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Monitoring the Reversibility of GPCR Signaling by Combining Photochromic Ligands with Label‐free Impedance Analysis ; day:18 ; month:04 ; year:2023 ; extent:12
Angewandte Chemie / International edition. International edition ; (18.04.2023) (gesamt 12)
- Urheber
-
Wirth, Ulrike
Erl, Julia
Azzam, Saphia
Höring, Carina
Skiba, Michael
Singh, Ritu
Hochmuth, Kathrin
Keller, Max
Wegener, Joachim
König, Burkhard
- DOI
-
10.1002/anie.202215547
- URN
-
urn:nbn:de:101:1-2023041915055410010402
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
14.08.2025, 10:58 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Wirth, Ulrike
- Erl, Julia
- Azzam, Saphia
- Höring, Carina
- Skiba, Michael
- Singh, Ritu
- Hochmuth, Kathrin
- Keller, Max
- Wegener, Joachim
- König, Burkhard
Ähnliche Objekte (12)
Monitoring the Reversibility of GPCR Signaling by Combining Photochromic Ligands with Label‐free Impedance Analysis
Development of Hybrid GPCR Ligands: Photochromic and Butyrylcholinesterase Inhibiting Human Cannabinoid Receptor 2 Agonists
Label-free monitoring of embolotherapy via catheter electrochemical impedance spectroscopy
Transepithelial Electrical Impedance Increase Following Porous Substrate Electroporation Enables Label‐Free Delivery
Functionalized Photochromic Scaffolds
Thermodynamics of GPCR activation
Perspective on photochromic compounds
Rapidly Switching Photochromic Dyes
Photochromic Nucleosides and Oligonucleotides
Photochromic Nucleosides and Oligonucleotides
On the Reversibility of Discretization
Proton transfer-mediated GPCR activation
Monitoring the Reversibility of GPCR Signaling by Combining Photochromic Ligands with Label‐free Impedance Analysis
Development of Hybrid GPCR Ligands: Photochromic and Butyrylcholinesterase Inhibiting Human Cannabinoid Receptor 2 Agonists
Label-free monitoring of embolotherapy via catheter electrochemical impedance spectroscopy
Transepithelial Electrical Impedance Increase Following Porous Substrate Electroporation Enables Label‐Free Delivery
Functionalized Photochromic Scaffolds
Thermodynamics of GPCR activation
Perspective on photochromic compounds
Rapidly Switching Photochromic Dyes
Photochromic Nucleosides and Oligonucleotides
Photochromic Nucleosides and Oligonucleotides
On the Reversibility of Discretization
Proton transfer-mediated GPCR activation
Monitoring the Reversibility of GPCR Signaling by Combining Photochromic Ligands with Label‐free Impedance Analysis
Development of Hybrid GPCR Ligands: Photochromic and Butyrylcholinesterase Inhibiting Human Cannabinoid Receptor 2 Agonists
Label-free monitoring of embolotherapy via catheter electrochemical impedance spectroscopy
Transepithelial Electrical Impedance Increase Following Porous Substrate Electroporation Enables Label‐Free Delivery
Functionalized Photochromic Scaffolds
Thermodynamics of GPCR activation
Perspective on photochromic compounds
Rapidly Switching Photochromic Dyes
Photochromic Nucleosides and Oligonucleotides
Photochromic Nucleosides and Oligonucleotides
On the Reversibility of Discretization