Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy
Abstract: The rhodamine system is a flexible framework for building small‐molecule fluorescent probes. Changing N‐substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si‐containing analogue of Q‐rhodamine. This probe is the first example of a “caged” Si‐rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red‐shifted to allow multicolor imaging. The dye is a useful label for super‐resolution imaging and constitutes a new scaffold for far‐red fluorogenic molecules.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy ; volume:55 ; number:5 ; year:2016 ; pages:1723-1727 ; extent:5
Angewandte Chemie / International edition. International edition ; 55, Heft 5 (2016), 1723-1727 (gesamt 5)
- Creator
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Grimm, Jonathan B.
Klein, Teresa
Kopek, Benjamin G.
Shtengel, Gleb
Hess, Harald F.
Sauer, Markus
Lavis, Luke D.
- DOI
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10.1002/anie.201509649
- URN
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urn:nbn:de:101:1-2022110104585617589196
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:37 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Grimm, Jonathan B.
- Klein, Teresa
- Kopek, Benjamin G.
- Shtengel, Gleb
- Hess, Harald F.
- Sauer, Markus
- Lavis, Luke D.
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