Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy
Abstract: The rhodamine system is a flexible framework for building small‐molecule fluorescent probes. Changing N‐substitution patterns and replacing the xanthene oxygen with a dimethylsilicon moiety can shift the absorption and fluorescence emission maxima of rhodamine dyes to longer wavelengths. Acylation of the rhodamine nitrogen atoms forces the molecule to adopt a nonfluorescent lactone form, providing a convenient method to make fluorogenic compounds. Herein, we take advantage of all of these structural manipulations and describe a novel photoactivatable fluorophore based on a Si‐containing analogue of Q‐rhodamine. This probe is the first example of a “caged” Si‐rhodamine, exhibits higher photon counts compared to established localization microscopy dyes, and is sufficiently red‐shifted to allow multicolor imaging. The dye is a useful label for super‐resolution imaging and constitutes a new scaffold for far‐red fluorogenic molecules.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy ; volume:55 ; number:5 ; year:2016 ; pages:1723-1727 ; extent:5
Angewandte Chemie / International edition. International edition ; 55, Heft 5 (2016), 1723-1727 (gesamt 5)
- Urheber
-
Grimm, Jonathan B.
Klein, Teresa
Kopek, Benjamin G.
Shtengel, Gleb
Hess, Harald F.
Sauer, Markus
Lavis, Luke D.
- DOI
-
10.1002/anie.201509649
- URN
-
urn:nbn:de:101:1-2022110104585617589196
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:37 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Grimm, Jonathan B.
- Klein, Teresa
- Kopek, Benjamin G.
- Shtengel, Gleb
- Hess, Harald F.
- Sauer, Markus
- Lavis, Luke D.
Ähnliche Objekte (12)
Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy
Expansion Microscopy combined with Super-Resolution Fluorescence Microscopy
Synthesis of a far-red photoactivatable silicon-containing rhodamine for super-resolution microscopy
Bioorthogonal labeling of neuronal proteins using super-resolution fluorescence microscopy
Super-Resolution Microscopy of Synaptic Proteins
Correlative live and fixed cell superresolution microscopy
Super-resolution microscopy of plasma membrane receptors
Super-resolution microscopy of the synaptic active zone
Quantitative Analysis of Membrane Components using Super-Resolution Microscopy
Single-molecule localization algorithms in super-resolution microscopy
Super-resolution microscopy of plasma membrane receptors and intracellular pathogens
Improving Super-Resolution Microscopy Data Reconstruction and Evaluation by Developing Advanced Processing Algorithms and Artifcial Neuronal Networks
Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy
Expansion Microscopy combined with Super-Resolution Fluorescence Microscopy
Synthesis of a far-red photoactivatable silicon-containing rhodamine for super-resolution microscopy
Bioorthogonal labeling of neuronal proteins using super-resolution fluorescence microscopy
Super-Resolution Microscopy of Synaptic Proteins
Correlative live and fixed cell superresolution microscopy
Super-resolution microscopy of plasma membrane receptors
Super-resolution microscopy of the synaptic active zone
Quantitative Analysis of Membrane Components using Super-Resolution Microscopy
Single-molecule localization algorithms in super-resolution microscopy
Super-resolution microscopy of plasma membrane receptors and intracellular pathogens
Improving Super-Resolution Microscopy Data Reconstruction and Evaluation by Developing Advanced Processing Algorithms and Artifcial Neuronal Networks
Synthesis of a Far‐Red Photoactivatable Silicon‐Containing Rhodamine for Super‐Resolution Microscopy
Expansion Microscopy combined with Super-Resolution Fluorescence Microscopy
Synthesis of a far-red photoactivatable silicon-containing rhodamine for super-resolution microscopy
Bioorthogonal labeling of neuronal proteins using super-resolution fluorescence microscopy
Super-Resolution Microscopy of Synaptic Proteins
Correlative live and fixed cell superresolution microscopy
Super-resolution microscopy of plasma membrane receptors
Super-resolution microscopy of the synaptic active zone
Quantitative Analysis of Membrane Components using Super-Resolution Microscopy
Single-molecule localization algorithms in super-resolution microscopy
Super-resolution microscopy of plasma membrane receptors and intracellular pathogens