Identification of XylR, the activator of Arabinose/Xylose inducible regulon in sulfolobus acidocaldarius and its application for homologous protein expression
Abstract: The thermophilic archaeon Sulfolobus acidocaldarius can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR (saci_2116) responsible for the transcriptional regulation of the pentose transporter and pentose metabolizing genes in S. acidocaldarius. A xylR deletion mutant showed growth retardation on D-xylose/L-arabinose containing media and the lack of transcription of the respective ABC transporter. In contrast to so far used promoters for expression in S. acidocaldarius, the xylR responsive promoters have a very low background activity. Finally, two XylR dependent promoters next to the long-established maltose inducible promotor were used to construct a high-throughput expression vector system for S. acidocaldarius to efficiently clone and express proteins in S. acidocaldarius
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Frontiers in microbiology. - 11 (2020) , 01066, ISSN: 1664-302X
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2020
- Urheber
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Kolk, Nienke van der
Wagner, Alexander
Wagner, Michaela
Waßmer, Bianca
Siebers, Bettina
Albers, Sonja Verena
- DOI
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10.3389/fmicb.2020.01066
- URN
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urn:nbn:de:bsz:25-freidok-1727834
- Rechteinformation
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 10:44 MESZ
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Beteiligte
- Kolk, Nienke van der
- Wagner, Alexander
- Wagner, Michaela
- Waßmer, Bianca
- Siebers, Bettina
- Albers, Sonja Verena
- Universität
Entstanden
- 2020
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