Identification of XylR, the activator of Arabinose/Xylose inducible regulon in sulfolobus acidocaldarius and its application for homologous protein expression
Abstract: The thermophilic archaeon Sulfolobus acidocaldarius can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR (saci_2116) responsible for the transcriptional regulation of the pentose transporter and pentose metabolizing genes in S. acidocaldarius. A xylR deletion mutant showed growth retardation on D-xylose/L-arabinose containing media and the lack of transcription of the respective ABC transporter. In contrast to so far used promoters for expression in S. acidocaldarius, the xylR responsive promoters have a very low background activity. Finally, two XylR dependent promoters next to the long-established maltose inducible promotor were used to construct a high-throughput expression vector system for S. acidocaldarius to efficiently clone and express proteins in S. acidocaldarius
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Frontiers in microbiology. - 11 (2020) , 01066, ISSN: 1664-302X
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2020
- Creator
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Kolk, Nienke van der
Wagner, Alexander
Wagner, Michaela
Waßmer, Bianca
Siebers, Bettina
Albers, Sonja Verena
- DOI
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10.3389/fmicb.2020.01066
- URN
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urn:nbn:de:bsz:25-freidok-1727834
- Rights
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:44 AM CEST
Data provider
This object is provided by:
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kolk, Nienke van der
- Wagner, Alexander
- Wagner, Michaela
- Waßmer, Bianca
- Siebers, Bettina
- Albers, Sonja Verena
- Universität
Time of origin
- 2020
Other Objects (12)
Two membrane-bound transcription factors regulate expression of various type-IV-pili surface structures in Sulfolobus acidocaldarius
Optimizing the genetic toolbox of the crenarchaeon Sulfolobus acidocaldarius for use in industrial applications
Adhesion pilus retraction powers twitching motility in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius
SaUspA, the universal stress protein of Sulfolobus acidocaldarius stimulates the activity of the PP2A phosphatase and is involved in growth at high salinity
Putative nucleotide-based second messengers in the archaeal model organisms Haloferax volcanii and Sulfolobus acidocaldarius
AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7
The use of thermostable fluorescent proteins for live imaging in Sulfolobus acidocaldarius
A regulatory RNA is involved in RNA duplex formation and biofilm regulation in Sulfolobus acidocaldarius
AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7
Application of the endogenous CRISPR-Cas type I-D system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocaldarius
Role of VapBC4 toxin-antitoxin system of Sulfolobus acidocaldarius in heat stress adaptation
Early response of Sulfolobus acidocaldarius to nutrient limitation
Two membrane-bound transcription factors regulate expression of various type-IV-pili surface structures in Sulfolobus acidocaldarius
Optimizing the genetic toolbox of the crenarchaeon Sulfolobus acidocaldarius for use in industrial applications
Adhesion pilus retraction powers twitching motility in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius
SaUspA, the universal stress protein of Sulfolobus acidocaldarius stimulates the activity of the PP2A phosphatase and is involved in growth at high salinity
Putative nucleotide-based second messengers in the archaeal model organisms Haloferax volcanii and Sulfolobus acidocaldarius
AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7
The use of thermostable fluorescent proteins for live imaging in Sulfolobus acidocaldarius
A regulatory RNA is involved in RNA duplex formation and biofilm regulation in Sulfolobus acidocaldarius
AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7
Application of the endogenous CRISPR-Cas type I-D system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocaldarius
Role of VapBC4 toxin-antitoxin system of Sulfolobus acidocaldarius in heat stress adaptation
Early response of Sulfolobus acidocaldarius to nutrient limitation
Two membrane-bound transcription factors regulate expression of various type-IV-pili surface structures in Sulfolobus acidocaldarius
Optimizing the genetic toolbox of the crenarchaeon Sulfolobus acidocaldarius for use in industrial applications
Adhesion pilus retraction powers twitching motility in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius
SaUspA, the universal stress protein of Sulfolobus acidocaldarius stimulates the activity of the PP2A phosphatase and is involved in growth at high salinity
Putative nucleotide-based second messengers in the archaeal model organisms Haloferax volcanii and Sulfolobus acidocaldarius
AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7
The use of thermostable fluorescent proteins for live imaging in Sulfolobus acidocaldarius
A regulatory RNA is involved in RNA duplex formation and biofilm regulation in Sulfolobus acidocaldarius
AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7
Application of the endogenous CRISPR-Cas type I-D system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocaldarius
Role of VapBC4 toxin-antitoxin system of Sulfolobus acidocaldarius in heat stress adaptation