Analysis of slow-cycling variants of the light-inducible nuclear protein export system LEXY in mammalian cells
Abstract: The optogenetic tool LEXY consists of the second light oxygen voltage (LOV) domain of Avena sativa phototropin 1 mutated to contain a nuclear export signal. It allows exporting from the nucleus with blue light proteins of interest (POIs) genetically fused to it. Mutations slowing the dark recovery rate of the LOV domain within LEXY were recently shown to allow for better depletion of some POIs from the nucleus in Drosophila embryos and for the usage of low light illumination regimes. We investigated these variants in mammalian cells and found they increase the cytoplasmic localization of the proteins we tested after illumination, but also during the dark phases, which corresponds to higher leakiness of the system. These data suggest that, when aiming to sequester into the nucleus a protein with a cytoplasmic function, the original LEXY is preferable. The iLEXY variants are, instead, advantageous when wanting to deplete the nucleus of the POI as much as possible
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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ACS synthetic biology. - 11, 10 (2022) , 3529-3533, ISSN: 2161-5063
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2022
- Urheber
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Forlani, Giada
Antwi, Enoch
Weis, Daniel Georg
Öztürk, Mehmet Ali
Queck, Bastian A.W
Brecht, Dominik
Di Ventura, Barbara
- Beteiligte Personen und Organisationen
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Biologische Signalforschung
- DOI
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10.1021/acssynbio.2c00232
- URN
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urn:nbn:de:bsz:25-freidok-2300590
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:56 MEZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Forlani, Giada
- Antwi, Enoch
- Weis, Daniel Georg
- Öztürk, Mehmet Ali
- Queck, Bastian A.W
- Brecht, Dominik
- Di Ventura, Barbara
- Biologische Signalforschung
- Universität
Entstanden
- 2022
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