Squaramides and Ureas: A Flexible Approach to Polymerase‐Compatible Nucleic Acid Assembly

to related objects

Abstract: Joining oligonucleotides together (ligation) is a powerful means of retrieving information from the nanoscale. To recover this information, the linkages created must be compatible with polymerases. However, enzymatic ligation is restrictive and current chemical ligation methods lack flexibility. Herein, a versatile ligation platform based on the formation of urea and squaramide artificial backbones from minimally modified 3′‐ and 5′‐amino oligonucleotides is described. One‐pot ligation gives a urea linkage with excellent read‐through speed, or a squaramide linkage that is read‐through under selective conditions. The squaramide linkage can be broken and reformed on demand, while stable pre‐activated precursor oligonucleotides expand the scope of the ligation reaction to reagent‐free, mild conditions. The utility of our system is demonstrated by replacing the enzymatically biased RNA‐to‐DNA reverse transcription step of RT‐qPCR with a rapid nucleic‐acid‐template‐dependent DNA chemical ligation system, that allows direct RNA detection.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Squaramides and Ureas: A Flexible Approach to Polymerase‐Compatible Nucleic Acid Assembly ; volume:59 ; number:28 ; year:2020 ; pages:11416-11422 ; extent:7
Angewandte Chemie / International edition. International edition ; 59, Heft 28 (2020), 11416-11422 (gesamt 7)

Creator: Shivalingam, Arun
Taemaitree, Lapatrada
El‐Sagheer, Afaf H.
Brown, Tom

DOI: 10.1002/anie.202000209

URN: urn:nbn:de:101:1-2022061113045112595933

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:34 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.

Show original at data provider

Associated

Shivalingam, Arun
Taemaitree, Lapatrada
El‐Sagheer, Afaf H.
Brown, Tom

Other Objects (12)

Squaramides and Ureas: A Flexible Approach to Polymerase‐Compatible Nucleic Acid Assembly

Squaramides and Ureas: A Flexible Approach to Polymerase‐Compatible Nucleic Acid Assembly

Flexible assembly systems

Flexible assembly systems

Cooperative nucleic acid binding by Poly ADP-ribose polymerase 1

Cooperative nucleic acid binding by Poly ADP-ribose polymerase 1

Self-assembly of proteins and their nucleic acids

Self-assembly of proteins and their nucleic acids

Nucleic acid-mediated fluorescence activation and chromophore assembly

Nucleic acid-mediated fluorescence activation and chromophore assembly

Aqueous Compatible Post‐Synthetic On‐Column Conjugation of Nucleic Acids Using Amino‐Modifiers

Aqueous Compatible Post‐Synthetic On‐Column Conjugation of Nucleic Acids Using Amino‐Modifiers

Display of functional nucleic acid polymerase on Escherichia coli surface and its application in directed polymerase evolution

Display of functional nucleic acid polymerase on Escherichia coli surface and its application in directed polymerase evolution

Flexible and three-dimensional CMOS-compatible intracerebral neural probes

Flexible and three-dimensional CMOS-compatible intracerebral neural probes

Bound-free phase detection immunoassay compatible with nucleic acid amplification point-of-care devices

Hochschulschrift

Bound-free phase detection immunoassay compatible with nucleic acid amplification point-of-care devices

Structural insights into a DNA polymerase reading the xeno nucleic acid HNA

Structural insights into a DNA polymerase reading the xeno nucleic acid HNA

Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

Nucleic acid nanostructures: From biomimetic assembly to in vivo applications

Nucleic acid nanostructures: From biomimetic assembly to in vivo applications

Squaramides and Ureas: A Flexible Approach to Polymerase‐Compatible Nucleic Acid Assembly

Squaramides and Ureas: A Flexible Approach to Polymerase‐Compatible Nucleic Acid Assembly

Flexible assembly systems

Flexible assembly systems

Cooperative nucleic acid binding by Poly ADP-ribose polymerase 1

Cooperative nucleic acid binding by Poly ADP-ribose polymerase 1

Self-assembly of proteins and their nucleic acids

Self-assembly of proteins and their nucleic acids

Nucleic acid-mediated fluorescence activation and chromophore assembly

Nucleic acid-mediated fluorescence activation and chromophore assembly

Aqueous Compatible Post‐Synthetic On‐Column Conjugation of Nucleic Acids Using Amino‐Modifiers

Aqueous Compatible Post‐Synthetic On‐Column Conjugation of Nucleic Acids Using Amino‐Modifiers

Display of functional nucleic acid polymerase on Escherichia coli surface and its application in directed polymerase evolution

Display of functional nucleic acid polymerase on Escherichia coli surface and its application in directed polymerase evolution

Flexible and three-dimensional CMOS-compatible intracerebral neural probes

Flexible and three-dimensional CMOS-compatible intracerebral neural probes

Bound-free phase detection immunoassay compatible with nucleic acid amplification point-of-care devices

Hochschulschrift

Bound-free phase detection immunoassay compatible with nucleic acid amplification point-of-care devices

Structural insights into a DNA polymerase reading the xeno nucleic acid HNA

Structural insights into a DNA polymerase reading the xeno nucleic acid HNA

Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

Nucleic acid nanostructures: From biomimetic assembly to in vivo applications

Nucleic acid nanostructures: From biomimetic assembly to in vivo applications

Squaramides and Ureas: A Flexible Approach to Polymerase‐Compatible Nucleic Acid Assembly

Squaramides and Ureas: A Flexible Approach to Polymerase‐Compatible Nucleic Acid Assembly

Flexible assembly systems

Flexible assembly systems

Cooperative nucleic acid binding by Poly ADP-ribose polymerase 1

Cooperative nucleic acid binding by Poly ADP-ribose polymerase 1

Self-assembly of proteins and their nucleic acids

Self-assembly of proteins and their nucleic acids

Nucleic acid-mediated fluorescence activation and chromophore assembly

Nucleic acid-mediated fluorescence activation and chromophore assembly

Aqueous Compatible Post‐Synthetic On‐Column Conjugation of Nucleic Acids Using Amino‐Modifiers

Aqueous Compatible Post‐Synthetic On‐Column Conjugation of Nucleic Acids Using Amino‐Modifiers

Display of functional nucleic acid polymerase on Escherichia coli surface and its application in directed polymerase evolution

Display of functional nucleic acid polymerase on Escherichia coli surface and its application in directed polymerase evolution

Flexible and three-dimensional CMOS-compatible intracerebral neural probes

Flexible and three-dimensional CMOS-compatible intracerebral neural probes

Bound-free phase detection immunoassay compatible with nucleic acid amplification point-of-care devices

Hochschulschrift

Bound-free phase detection immunoassay compatible with nucleic acid amplification point-of-care devices

Structural insights into a DNA polymerase reading the xeno nucleic acid HNA

Structural insights into a DNA polymerase reading the xeno nucleic acid HNA

Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase

Nucleic acid nanostructures: From biomimetic assembly to in vivo applications

Nucleic acid nanostructures: From biomimetic assembly to in vivo applications

Cultural heritage institutions wishing to register will find more information here.

Fields marked * need to be filled in.

Username*

Please enter your username

Email*

Please enter your email address

Please do not fill this field

First name

Last name

Password*

Please enter your password

Confirm password*

Please enter the same password

I have read the terms of use and the privacy policy for the collection of personal data and accept them. *

This field is required.

I would like to subscribe to the newsletter of the Deutsche Digitale Bibliothek. See newsletter subscription info.

Account created

Your "My DDB" account has been successfully created. Before you can log in to your account, you must click the confirmation link in the message we just sent to the email address you provided.