Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast
Abstract: The guided entry of tail-anchored proteins (GET) pathway assists in the posttranslational delivery of tail-anchored proteins, containing a single C-terminal transmembrane domain, to the ER. Here we uncover how the yeast GET pathway component Get4/5 facilitates capture of tail-anchored proteins by Sgt2, which interacts with tail-anchors and hands them over to the targeting component Get3. Get4/5 binds directly and with high affinity to ribosomes, positions Sgt2 close to the ribosomal tunnel exit, and facilitates the capture of tail-anchored proteins by Sgt2. The contact sites of Get4/5 on the ribosome overlap with those of SRP, the factor mediating cotranslational ER-targeting. Exposure of internal transmembrane domains at the tunnel exit induces high-affinity ribosome binding of SRP, which in turn prevents ribosome binding of Get4/5. In this way, the position of a transmembrane domain within nascent ER-targeted proteins mediates partitioning into either the GET or SRP pathway directly at the ribosomal tunnel exit
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Nature communications. - 12, 1 (2021) , 782, ISSN: 2041-1723
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2021
- Creator
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Zhang, Ying
Laurentiis, Evelina De
Bohnsack, Katherine E.
Wahlig, Mascha
Ranjan, Namit
Gruseck, Simon
Hackert, Philipp
Wölfle, Tina
Rodnina, Marina
Schwappach, Blanche
Rospert, Sabine
- DOI
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10.1038/s41467-021-20981-3
- URN
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urn:nbn:de:bsz:25-freidok-1935109
- Rights
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:42 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Zhang, Ying
- Laurentiis, Evelina De
- Bohnsack, Katherine E.
- Wahlig, Mascha
- Ranjan, Namit
- Gruseck, Simon
- Hackert, Philipp
- Wölfle, Tina
- Rodnina, Marina
- Schwappach, Blanche
- Rospert, Sabine
- Universität
Time of origin
- 2021
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Transport of Tail-anchored Proteins to the Inner Nuclear Membrane
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Pex19 and cytosolic Hsp70 are involved in the import of mitochondrial tail- anchored proteins
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Anchored inflation expectations
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