Ribosome-bound Get4/5 facilitates the capture of tail-anchored proteins by Sgt2 in yeast
Abstract: The guided entry of tail-anchored proteins (GET) pathway assists in the posttranslational delivery of tail-anchored proteins, containing a single C-terminal transmembrane domain, to the ER. Here we uncover how the yeast GET pathway component Get4/5 facilitates capture of tail-anchored proteins by Sgt2, which interacts with tail-anchors and hands them over to the targeting component Get3. Get4/5 binds directly and with high affinity to ribosomes, positions Sgt2 close to the ribosomal tunnel exit, and facilitates the capture of tail-anchored proteins by Sgt2. The contact sites of Get4/5 on the ribosome overlap with those of SRP, the factor mediating cotranslational ER-targeting. Exposure of internal transmembrane domains at the tunnel exit induces high-affinity ribosome binding of SRP, which in turn prevents ribosome binding of Get4/5. In this way, the position of a transmembrane domain within nascent ER-targeted proteins mediates partitioning into either the GET or SRP pathway directly at the ribosomal tunnel exit
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Nature communications. - 12, 1 (2021) , 782, ISSN: 2041-1723
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2021
- Urheber
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Zhang, Ying
Laurentiis, Evelina De
Bohnsack, Katherine E.
Wahlig, Mascha
Ranjan, Namit
Gruseck, Simon
Hackert, Philipp
Wölfle, Tina
Rodnina, Marina
Schwappach, Blanche
Rospert, Sabine
- DOI
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10.1038/s41467-021-20981-3
- URN
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urn:nbn:de:bsz:25-freidok-1935109
- Rechteinformation
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:32 MESZ
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Beteiligte
- Zhang, Ying
- Laurentiis, Evelina De
- Bohnsack, Katherine E.
- Wahlig, Mascha
- Ranjan, Namit
- Gruseck, Simon
- Hackert, Philipp
- Wölfle, Tina
- Rodnina, Marina
- Schwappach, Blanche
- Rospert, Sabine
- Universität
Entstanden
- 2021
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