RIP-Seq suggests translational regulation by L7Ae in Archaea
Abstract: L7Ae is a universal archaeal protein that recognizes and stabilizes kink-turn (k-turn) motifs in RNA substrates. These structural motifs are widespread in na-ture and are found in many functional RNA species, including ribosomal RNAs. Syn-thetic biology approaches utilize L7Ae/k-turn interactions to control gene expressionin eukaryotes. Here, we present results of comprehensive RNA immunoprecipitationsequencing (RIP-Seq) analysis of genomically tagged L7Ae from the hyperthermo-philic archaeonSulfolobus acidocaldarius. A large set of interacting noncoding RNAswas identified. In addition, several mRNAs, including thel7aetranscript, were foundto contain k-turn motifs that facilitate L7Ae binding.In vivostudies showed thatL7Ae autoregulates the translation of its mRNA by binding to a k-turn motif presentin the 5=untranslated region (UTR). A green fluorescent protein (GFP) reporter sys-tem was established inEscherichia coliand verified conservation of L7Ae-mediatedfeedback regulation inArchaea. Mobility shift assays confirmed binding to a k-turnin the transcript ofnop5-fibrillarin, suggesting that the expression of all C/D boxsRNP core proteins is regulated by L7Ae. These studies revealed that L7Ae-mediatedgene regulation evolved in archaeal organisms, generating new tools for the modu-lation of synthetic gene circuits in bacteria
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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mBio. - 8, 4 (2017) , e00730-17, ISSN: 2150-7511
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2019
- Creator
- DOI
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10.1128/mbio.00730-17
- URN
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urn:nbn:de:bsz:25-freidok-1438360
- Rights
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Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:51 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Daume, Michael
- Uhl, Michael
- Backofen, Rolf
- Randau, Lennart
- Universität
Time of origin
- 2019
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