Cyclic stretch increases splicing noise rate in cultured human fibroblasts

Abstract: Background
Mechanical forces are known to alter the expression of genes, but it has so far not been reported whether they may influence the fidelity of nucleus-based processes. One experimental approach permitting to address this question is the application of cyclic stretch to cultured human fibroblasts. As a marker for the precision of nucleus-based processes, the number of errors that occur during co-transcriptional splicing can then be measured. This so-called splicing noise is found at low frequency in pre-mRNA splicing.

Findings
The amount of splicing noise was measured by RT-qPCR of seven exon skips from the test genes AATF, MAP3K11, NF1, PCGF2, POLR2A and RABAC1. In cells treated by altered uniaxial cyclic stretching for 18 h, a uniform and significant increase of splicing noise was found for all detectable exon skips.

Conclusion
Our data demonstrate that application of cyclic stretch to cultured fibroblasts correlates with a reduced transcriptional fidelity caused by increasing splicing noise

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: BMC research notes. - 4, 1 (2011) , 470, ISSN: 1756-0500

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2022
• Creator: Uhl, Michael ; Mellert, Kevin Marcel ; Striegl, Britta ; Deibler, Martin ; Lamla, Markus ; Spatz, Joachim P. ; Kemkemer, Ralf ; Kaufmann, Dieter

Further information

• DOI: 10.1186/1756-0500-4-470
• URN: urn:nbn:de:bsz:25-freidok-2320577
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:48 PM CET