Single-cell RNA-seq reveals that CD9 Is a negative marker of glucose-responsive pancreatic [beta]-like cells derived from human pluripotent stem cells
Abstract: To date, it remains unclear if there are specific cell-surface markers for purifying glucose-responsive pancreatic β-like cells derived from human pluripotent stem cells (hPSCs). In searching for this, we generated an efficient protocol for differentiating β-like cells from human embryonic stem cells. We performed single-cell RNA sequencing and found that CD9 is a negative cell-surface marker of β-like cells, as most INS+ cells are CD9−. We purified β-like cells for spontaneous formation of islet-like organoids against CD9, and found significantly more NKX6.1+MAFA+C-PEPTIDE+ β-like cells in the CD9− than in the CD9+ population. CD9− cells also demonstrate better glucose responsiveness than CD9+ cells. In humans, we observe more CD9+C-PEPTIDE+ β cells in the fetal than in the adult cadaveric islets and more Ki67+ proliferating cells among CD9+ fetal β cells. Taken together, our experiments show that CD9 is a cell-surface marker for negative enrichment of glucose-responsive β-like cells differentiated from hPSCs
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Stem cell reports. - 15, 5 (2020) , 1111-1126, ISSN: 2213-6711
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2020
- Urheber
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Li, Xisheng
Yang, Kevin Y.
Chan, Vicken W.
Leung, Kam Tong
Zhang, Xiao-Bing
Wong, Alan S.
Chong, Charing C. N.
Wang, Chi Chiu
Ku, Manching
Lui, Kathy O.
- DOI
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10.1016/j.stemcr.2020.09.009
- URN
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urn:nbn:de:bsz:25-freidok-1697466
- Rechteinformation
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:49 MEZ
Datenpartner
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Beteiligte
- Li, Xisheng
- Yang, Kevin Y.
- Chan, Vicken W.
- Leung, Kam Tong
- Zhang, Xiao-Bing
- Wong, Alan S.
- Chong, Charing C. N.
- Wang, Chi Chiu
- Ku, Manching
- Lui, Kathy O.
- Universität
Entstanden
- 2020
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