Cohesin contributes to transcriptional repression of stage‐specific genes in the human malaria parasite
Abstract: The complex life cycle of the human malaria parasite, Plasmodium falciparum, is driven by specific transcriptional programs, but it is unclear how most genes are activated or silenced at specific times. There is an association between transcription and spatial organization; however, the molecular mechanisms behind genome organization are unclear. While P. falciparum lacks key genome‐organizing proteins found in metazoans, it has all core components of the cohesin complex. To investigate the role of cohesin in P. falciparum, we functionally characterize the cohesin subunit Structural Maintenance of Chromosomes protein 3 (SMC3). SMC3 knockdown during early stages of the intraerythrocytic developmental cycle (IDC) upregulates a subset of genes involved in erythrocyte egress and invasion, which are normally expressed at later stages. ChIP‐seq analyses reveal that during the IDC, SMC3 enrichment at the promoter regions of these genes inversely correlates with gene expression and chromatin accessibility. These data suggest that SMC3 binding contributes to the repression of specific genes until their appropriate time of expression, revealing a new mode of stage‐specific gene repression in P. falciparum.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Cohesin contributes to transcriptional repression of stage‐specific genes in the human malaria parasite ; day:18 ; month:08 ; year:2023 ; extent:20
EMBO reports / European Molecular Biology Organization ; (18.08.2023) (gesamt 20)
- Creator
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Rosa, Catarina
Singh, Parul
Chen, Patty
Sinha, Ameya
Claës, Aurélie
Preiser, Peter R.
Dedon, Peter C.
Baumgarten, Sebastian
Scherf, Artur
Bryant, Jessica M.
- DOI
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10.15252/embr.202357090
- URN
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urn:nbn:de:101:1-2023081815341198565499
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:55 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Rosa, Catarina
- Singh, Parul
- Chen, Patty
- Sinha, Ameya
- Claës, Aurélie
- Preiser, Peter R.
- Dedon, Peter C.
- Baumgarten, Sebastian
- Scherf, Artur
- Bryant, Jessica M.
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Stage-specific detection and characterisation of the malaria parasite
Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella
Overexpression of the cohesin-core subunit SMC1A contributes to colorectal cancer development
Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2
A new meiosis‐specific cohesin complex implicated in the cohesin code for homologous pairing
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Cohesin prevents cross-domain gene coactivation
Mechanical disengagement of the cohesin ring
Ontogenetic stage-specific reciprocal intraguild predation
Stage-specific miRNAs regulate gene expression associated with growth, development and parasite-host interaction during the intra-mammalian migration of the zoonotic helminth parasite Fasciola hepatica
The dual use of cohesin and its protector Sgo1 contributes to the choreography of the chromosome and the centrosome cycle
Stage-specific binding profile of cohesin in resting and switch-activated B lymphocytes
Stage-specific detection and characterisation of the malaria parasite
Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella
Overexpression of the cohesin-core subunit SMC1A contributes to colorectal cancer development
Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2
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Ontogenetic stage-specific reciprocal intraguild predation
Stage-specific miRNAs regulate gene expression associated with growth, development and parasite-host interaction during the intra-mammalian migration of the zoonotic helminth parasite Fasciola hepatica