Spatially‐Resolved Organoid Transfection by Porous Silicon‐Mediated Optoporation

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Abstract: Engineering the spatial organisation of organotypic cultures is pivotal for refining tissue models that are useful for gaining deeper insights into complex, non‐cell autonomous processes. These advanced models are key to improving the understanding of fundamental biological mechanisms and therapeutic strategies. Controlling gene regulation through spatially‐resolved delivery of nucleic acids provides an attractive approach to produce such tissue models. An emerging strategy for spatially‐resolved transfection uses photosensitizing nanoparticles coupled with laser pulses to optoporate cells in culture and locally mediate gene delivery. However, localized optoporation in 3D systems remains challenging. Here we propose a solution to this longstanding hurdle, demonstrating that porous silicon nanoparticles are a safe and bioresorbable photosensitising nanomaterial capable of spatially‐resolved transfection of mRNA in MCF‐7 organoids by near‐infrared two‐photon optoporation. Functionalization with an azobenzene–lysine photo‐switchable moiety enhances the transfection efficiency of the nanoparticles up to 84% in a 2D cell system. Moreover, the nanoparticles enable spatially selective mRNA transfection to MCF‐7 spheroids, demonstrating targeted gene delivery in complex 3D cellular environments. The approach for spatially‐resolved 3D optoporation offers a way forward for the design of tailored spheroids and organoids by spatially selective nucleic acids delivery.

Standort: Deutsche Nationalbibliothek Frankfurt am Main

Umfang: Online-Ressource

Sprache: Englisch

Erschienen in: Spatially‐Resolved Organoid Transfection by Porous Silicon‐Mediated Optoporation ; day:17 ; month:10 ; year:2024 ; extent:15
Advanced materials ; (17.10.2024) (gesamt 15)

Urheber: Spiteri, Chantelle
Caprettini, Valeria
Wang, Yikai
Dominguez‐Gil, Sofia
Kaasalainen, Martti
Wang, Cong
Martella, Davide Alessandro
McLennan, Samuel
Vashisth, Priya
Gary‐Bobo, Magali
Nguyen, Christophe
Bergholt, Mads
Durand, Jean‐Olivier
Cunin, Frédérique
Chiappini, Ciro

DOI: 10.1002/adma.202407650

URN: urn:nbn:de:101:1-2410181446372.801943550711

Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Letzte Aktualisierung: 15.08.2025, 07:20 MESZ

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Beteiligte

Spiteri, Chantelle
Caprettini, Valeria
Wang, Yikai
Dominguez‐Gil, Sofia
Kaasalainen, Martti
Wang, Cong
Martella, Davide Alessandro
McLennan, Samuel
Vashisth, Priya
Gary‐Bobo, Magali
Nguyen, Christophe
Bergholt, Mads
Durand, Jean‐Olivier
Cunin, Frédérique
Chiappini, Ciro

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