Stress‐dependent macromolecular crowding in the mitochondrial matrix
Abstract: Macromolecules of various sizes induce crowding of the cellular environment. This crowding impacts on biochemical reactions by increasing solvent viscosity, decreasing the water‐accessible volume and altering protein shape, function, and interactions. Although mitochondria represent highly protein‐rich organelles, most of these proteins are somehow immobilized. Therefore, whether the mitochondrial matrix solvent exhibits macromolecular crowding is still unclear. Here, we demonstrate that fluorescent protein fusion peptides (AcGFP1 concatemers) in the mitochondrial matrix of HeLa cells display an elongated molecular structure and that their diffusion constant decreases with increasing molecular weight in a manner typical of macromolecular crowding. Chloramphenicol (CAP) treatment impaired mitochondrial function and reduced the number of cristae without triggering mitochondrial orthodox‐to‐condensed transition or a mitochondrial unfolded protein response. CAP‐treated cells displayed progressive concatemer immobilization with increasing molecular weight and an eightfold matrix viscosity increase, compatible with increased macromolecular crowding. These results establish that the matrix solvent exhibits macromolecular crowding in functional and dysfunctional mitochondria. Therefore, changes in matrix crowding likely affect matrix biochemical reactions in a manner depending on the molecular weight of the involved crowders and reactants.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Stress‐dependent macromolecular crowding in the mitochondrial matrix ; day:24 ; month:02 ; year:2023 ; extent:24
The EMBO journal / European Molecular Biology Organization ; (24.02.2023) (gesamt 24)
- Urheber
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Bulthuis, Elianne P.
Dieteren, Cindy E. J.
Bergmans, Jesper
Berkhout, Job
Wagenaars, Jori A.
van de Westerlo, Els M. A.
Podhumljak, Emina
Hink, Mark A.
Hesp, Laura F. B.
Rosa, Hannah S.
Malik, Afshan N.
Lindert, Mariska Kea‐te
Willems, Peter H. G. M.
Gardeniers, Han
den Otter, Wouter K.
Adjobo‐Hermans, Merel J. W.
Koopman, Werner J. H.
- DOI
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10.15252/embj.2021108533
- URN
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urn:nbn:de:101:1-2023022414285931228835
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 10:56 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Bulthuis, Elianne P.
- Dieteren, Cindy E. J.
- Bergmans, Jesper
- Berkhout, Job
- Wagenaars, Jori A.
- van de Westerlo, Els M. A.
- Podhumljak, Emina
- Hink, Mark A.
- Hesp, Laura F. B.
- Rosa, Hannah S.
- Malik, Afshan N.
- Lindert, Mariska Kea‐te
- Willems, Peter H. G. M.
- Gardeniers, Han
- den Otter, Wouter K.
- Adjobo‐Hermans, Merel J. W.
- Koopman, Werner J. H.
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