High Macromolecular Crowding in Liposomes from Microfluidics

Abstract: The intracellular environment is crowded with macromolecules that influence biochemical equilibria and biomacromolecule diffusion. The incorporation of such crowding in synthetic cells would be needed to mimic the biochemistry of living cells. However, only a few methods provide crowded artificial cells, moreover providing cells with either heterogeneous size and composition or containing a significant oil fraction. Therefore, a method that generates monodisperse liposomes with minimal oil content and tunable macromolecular crowding using polydimethylsiloxane (PDMS)‐based microfluidics is presented. Lipid stabilized water‐in‐oil‐in‐water emulsions that are stable for at least several months and with a high macromolecular crowder concentration that can be controlled with the external osmolality are formed. A crucial feature is that the oil phase can be removed using high flow conditions at any point after production, providing the highly crowded liposomes. Genetically encoded macromolecular crowding sensors show that the high level of macromolecular crowding in the emulsions is fully retained throughout the generation of minimal‐oil lipid bilayers. This modular and robust platform will serve the study of biochemistry under physiologically relevant crowding conditions.

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• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: High Macromolecular Crowding in Liposomes from Microfluidics ; day:29 ; month:07 ; year:2022 ; extent:12 ; Advanced science ; (29.07.2022) (gesamt 12)

Contributors, Places and Time

• Creator: Guerzoni, Luis P. B. ; de Goes, André V. C. ; Kalacheva, Milara ; Haduła, Jakub ; Mork, Matthias ; De Laporte, Laura ; Boersma, Arnold J.

Further information

• DOI: 10.1002/advs.202201169
• URN: urn:nbn:de:101:1-2022072915235062227150
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.