Catalytic activity of glucose oxidase after dielectrophoretic immobilization on nanoelectrodes

Abstract: Dielectrophoresis (DEP) is an AC electrokinetic effect that is proven to be effective for the immobilization of not only cells, but also of macromolecules, for example, antibodies and enzyme molecules. In our previous work, we have already demonstrated the high catalytic activity of immobilized horseradish peroxidase after DEP. To evaluate the suitability of the immobilization method for sensing or research in general, we want to test it for other enzymes, too. In this study, glucose oxidase (GOX) from Aspergillus niger was immobilized on TiN nanoelectrode arrays by DEP. Fluorescence microscopy showed the intrinsic fluorescence of the immobilized enzymes flavin cofactor on the electrodes. The catalytic activity of immobilized GOX was detectable, but a fraction of less than 1.3% of the maximum activity that was expected for a full monolayer of immobilized enzymes on all electrodes was stable for multiple measurement cycles. Therefore, the effect of DEP immobilization on the catalytic activity strongly depends on the used enzyme.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Catalytic activity of glucose oxidase after dielectrophoretic immobilization on nanoelectrodes ; day:16 ; month:03 ; year:2023 ; extent:12 ; Electrophoresis ; (16.03.2023) (gesamt 12)

Beteiligte, Orts- und Zeitangaben

• Urheber: Prüfer, Mareike ; Stanke, Sandra ; Bier, Frank F. ; Hölzel, Ralph

Weitere Informationen

• DOI: 10.1002/elps.202300010
• URN: urn:nbn:de:101:1-2023031714151481238059
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 14.08.2025, 10:44 MESZ