Coacervate Droplets for Synthetic Cells

Abstract: The design and construction of synthetic cells – human‐made microcompartments that mimic features of living cells – have experienced a real boom in the past decade. While many efforts have been geared toward assembling membrane‐bounded compartments, coacervate droplets produced by liquid–liquid phase separation have emerged as an alternative membrane‐free compartmentalization paradigm. Here, the dual role of coacervate droplets in synthetic cell research is discussed: encapsulated within membrane‐enclosed compartments, coacervates act as surrogates of membraneless organelles ubiquitously found in living cells; alternatively, they can be viewed as crowded cytosol‐like chassis for constructing integrated synthetic cells. After introducing key concepts of coacervation and illustrating the chemical diversity of coacervate systems, their physicochemical properties and resulting bioinspired functions are emphasized. Moving from suspensions of free floating coacervates, the two nascent roles of these droplets in synthetic cell research are highlighted: organelle‐like modules and cytosol‐like templates. Building the discussion on recent studies from the literature, the potential of coacervate droplets to assemble integrated synthetic cells capable of multiple life‐inspired functions is showcased. Future challenges that are still to be tackled in the field are finally discussed.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Coacervate Droplets for Synthetic Cells ; day:18 ; month:07 ; year:2023 ; extent:26 ; Small Methods ; (18.07.2023) (gesamt 26)

Contributors, Places and Time

• Creator: Lin, Zi ; Beneyton, Thomas ; Baret, Jean‐Christophe ; Martin, Nicolas

Further information

• DOI: 10.1002/smtd.202300496
• URN: urn:nbn:de:101:1-2023071815221639315119
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 11:00 AM CEST