Synthetic Membraneless Droplets for Synaptic‐Like Clustering of Lipid Vesicles

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Abstract: In eukaryotic cells, the membraneless organelles (MLOs) formed via liquid‐liquid phase separation (LLPS) are found to interact intimately with membranous organelles (MOs). One major mode is the clustering of MOs by MLOs, such as the formation of clusters of synaptic vesicles at nerve terminals mediated by the synapsin‐rich MLOs. Aqueous droplets, including complex coacervates and aqueous two‐phase systems, have been plausible MLO‐mimics to emulate or elucidate biological processes. However, neither of them can cluster lipid vesicles (LVs) like MLOs. In this work, we develop a synthetic droplet assembled from a combination of two different interactions underlying the formation of these two droplets, namely, associative and segregative interactions, which we call segregative‐associative (SA) droplets. The SA droplets cluster and disperse LVs recapitulating the key functional features of synapsin condensates, which can be attributed to the weak electrostatic interaction environment provided by SA droplets. This work suggests LLPS with combined segregative and associative interactions as a possible route for synaptic clustering of lipid vesicles and highlights SA droplets as plausible MLO‐mimics and models for studying and mimicking related cellular dynamics.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Synthetic Membraneless Droplets for Synaptic‐Like Clustering of Lipid Vesicles ; day:06 ; month:10 ; year:2023 ; extent:10
Angewandte Chemie ; (06.10.2023) (gesamt 10)

Creator: Li, Qingchuan
Song, Qingchun
Guo, Wei
Cao, Yang
Cui, Xinyu
Chen, Dairong
Shum, Ho Cheung

DOI: 10.1002/ange.202313096

URN: urn:nbn:de:101:1-2023100615235962869013

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 14.08.2025, 10:55 AM CEST

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Li, Qingchuan
Song, Qingchun
Guo, Wei
Cao, Yang
Cui, Xinyu
Chen, Dairong
Shum, Ho Cheung

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