Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions

Abstract: Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI–Pc ternary complex, leading to exclusion of water molecules from PsaA–PsaB/Pc interface once the PSI–Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: Biochemical journal. - 478, 12 (2021) , 2371-2384, ISSN: 1470-8728

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2024
• Urheber: Caspy, Ido ; Fadeeva, Mariia ; Kuhlgert, Sebastian ; Borovikova-Sheinker, Anna ; Klaiman, Daniel ; Masrati, Gal ; Drepper, Friedel ; Ben-Tal, Nir ; Hippler, Michael ; Nelson, Nathan

Weitere Informationen

• DOI: 10.1042/bcj20210267
• URN: urn:nbn:de:bsz:25-freidok-2478630
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 14.08.2025, 10:45 MESZ