Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions
Abstract: Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 Å resolution reveals strong hydrophobic interactions in the plant PSI–Pc ternary complex, leading to exclusion of water molecules from PsaA–PsaB/Pc interface once the PSI–Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Biochemical journal. - 478, 12 (2021) , 2371-2384, ISSN: 1470-8728
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2024
- Urheber
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Caspy, Ido
Fadeeva, Mariia
Kuhlgert, Sebastian
Borovikova-Sheinker, Anna
Klaiman, Daniel
Masrati, Gal
Drepper, Friedel
Ben-Tal, Nir
Hippler, Michael
Nelson, Nathan
- DOI
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10.1042/bcj20210267
- URN
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urn:nbn:de:bsz:25-freidok-2478630
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:41 MEZ
Datenpartner
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Beteiligte
- Caspy, Ido
- Fadeeva, Mariia
- Kuhlgert, Sebastian
- Borovikova-Sheinker, Anna
- Klaiman, Daniel
- Masrati, Gal
- Drepper, Friedel
- Ben-Tal, Nir
- Hippler, Michael
- Nelson, Nathan
- Universität
Entstanden
- 2024