An improved organotypic cell culture system to study tissue-resident macrophages ex vivo

Abstract: Tissue-resident macrophages (TRMs) perform organ-specific functions that are dependent on factors such as hematopoietic origin, local environment, and biological influences. A diverse range of in vitro culture systems have been developed to decipher TRM functions, including bone marrow-derived macrophages (BMDMs), induced pluripotent stem cell (iPSC)-derived TRMs, or immortalized cell lines. However, despite the usefulness of such systems, there are notable limitations. Attempts to culture primary macrophages often require purification of cells and lack a high cell yield and consistent phenotype. Here, we aimed to address these limitations by establishing an organotypic primary cell culture protocol. We obtained long-term monocultures of macrophages derived from distinct organs without prior purification using specific growth factors and tissue normoxic conditions that largely conserved a TRM-like identity in vitro. Thus, this organotypic system offers an ideal screening platform for primary macrophages from different organs that can be used for a wide range of assays and readouts

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: Cell reports. Methods. - 2, 8 (2022) , 100260, ISSN: 2667-2375

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2022
• Creator: Aktories, Philipp J. K. ; Petry, Philippe ; Glatz, Paulo ; Geoffroy, Andrieux ; Oschwald, Alexander ; Botterer, Hannah ; Gorka, Oliver ; Erny, Daniel ; Börries, Melanie ; Henneke, Philipp ; Groß, Olaf ; Prinz, Marco ; Kierdorf, Katrin

Further information

• DOI: 10.1016/j.crmeth.2022.100260
• URN: urn:nbn:de:bsz:25-freidok-2294211
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:55 PM CET