Atrial-like engineered heart tissue: an in vitro model of the human atrium
Abstract: Cardiomyocytes (CMs) generated from human induced pluripotent stem cells (hiPSCs) are under investigation for their suitability as human models in preclinical drug development. Antiarrhythmic drug development focuses on atrial biology for the treatment of atrial fibrillation. Here we used recent retinoic acid-based protocols to generate atrial CMs from hiPSCs and establish right atrial engineered heart tissue (RA-EHT) as a 3D model of human atrium. EHT from standard protocol-derived hiPSC-CMs (Ctrl-EHT) and intact human muscle strips served as comparators. RA-EHT exhibited higher mRNA and protein concentrations of atrial-selective markers, faster contraction kinetics, lower force generation, shorter action potential duration, and higher repolarization fraction than Ctrl-EHTs. In addition, RA-EHTs but not Ctrl-EHTs responded to pharmacological manipulation of atrial-selective potassium currents. RA- and Ctrl-EHTs’ behavior reflected differences between human atrial and ventricular muscle preparations. Taken together, RA-EHT is a model of human atrium that may be useful in preclinical drug screening
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Stem cell reports. - 11, 6 (2018) , 1378-1390, ISSN: 2213-6711
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2019
- Urheber
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Lemme, Marta
Ulmer, Bärbel
Lemoine, Marc Daniel
Zech, Antonia T. L.
Flenner, Frederik
Ravens, Ursula
Reichenspurner, Hermann
Rol-Garcia, Miriam
Smith, Godfrey L.
Hansen, Arne
Christ, Torsten
Eschenhagen, Thomas
- DOI
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10.1016/j.stemcr.2018.10.008
- URN
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urn:nbn:de:bsz:25-freidok-1487762
- Rechteinformation
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:42 MEZ
Datenpartner
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Beteiligte
- Lemme, Marta
- Ulmer, Bärbel
- Lemoine, Marc Daniel
- Zech, Antonia T. L.
- Flenner, Frederik
- Ravens, Ursula
- Reichenspurner, Hermann
- Rol-Garcia, Miriam
- Smith, Godfrey L.
- Hansen, Arne
- Christ, Torsten
- Eschenhagen, Thomas
- Universität
Entstanden
- 2019