Development of a standardized and safe airborne antibacterial assay, and its evaluation on antibacterial biomimetic model surfaces

Abstract: Bacterial infection of biomaterials is a major concern in medicine, and different kinds of antimicrobial biomaterial have been developed to deal with this problem. To test the antimicrobial performance of these biomaterials, the airborne bacterial assay is used, which involves the formation of biohazardous bacterial aerosols. We here describe a new experimental set-up which allows safe handling of such pathogenic aerosols, and standardizes critical parameters of this otherwise intractable and strongly user-dependent assay. With this new method, reproducible, thorough antimicrobial data (number of colony forming units and live-dead-stain) was obtained. Poly(oxonorbornene)-based Synthetic Mimics of Antimicrobial Peptides (SMAMPs) were used as antimicrobial test samples. The assay was able to differentiate even between subtle sample differences, such as different sample thicknesses. With this new set-up, the airborne bacterial assay was thus established as a useful, reliable, and realistic experimental method to simulate the contamination of biomaterials with bacteria, for example in an intraoperative setting

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: PLoS ONE. 9, 10 (2014), e111357, DOI 10.1371/journal.pone.0111357, issn: 1932-6203 ; IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Classification and Topics

• Keyword: Aerosol ; Biomaterial ; Polymere ; Staphylococcus aureus ; Pathogene Bakterien ; Antimikrobieller Wirkstoff ; Glasur ; Kunststoff ; Biopharmazie ; Pharmakodynamik ; Pharmakokinetik

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2014
• Creator: Al-Ahmad, Ali ; Zou, Peng ; Guevara Solarte, Diana Lorena ; Hellwig, Elmar ; Steinberg, Thorsten ; Lienkamp, Karen
• Contributor: Freiburg Institute for Advanced Studies ; Albert-Ludwigs-Universität Freiburg ; Technische Fakultät ; Klinik für Zahnerhaltungskunde und Parodontologie ; Institut für Mikrosystemtechnik ; Medizinische Fakultät ; Abteilung für Orale Biotechnologie

Further information

• DOI: 10.1371/journal.pone.0111357
• URN: urn:nbn:de:bsz:25-freidok-120282
• Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:53 PM CET