hsa-miR-34a-5p enhances temozolomide anti-tumoral effects on glioblastoma : in-silico and in-vitro study

Abstract: Glioblastoma multiform (GBM) is a commonly diagnosed brain neoplasm with a poor prognosis. Accumulating evidence has highlighted the significance of microRNA (miR) dysregulation in tumor development and progression. This study investigated the effect of hsa-miR-34a-5p and its combination with temozolomide on GBM, the related molecular mechanisms, and the signaling pathway using in-silico and in-vitro approaches. The in-silico tumor bulk and single-cell RNA sequencing analyses were done on TCGA-GTEx, CGGA, GSE13276, GSE90603, and GSE182109 datasets. After selecting the A172 cell line, hsa-miR-34a-5p mimics were transfected, and the cell viability, migration, cell cycle, clonogenicity, and apoptosis of studied groups were studied using MTT, scratch, flow cytometry, colony formation, and Annexin V/PI assays. The mRNA expression of CASP9, CASP3, CASP8, MMP2, CD44, CDK6, CDK4, CCND1, RAF1, MAP2K1, MET, SRC, and CD274 was studied using qRT-PCR method. hsa-miR-34a-5p downregulated RAF1 ex.... https://www.excli.de/index.php/excli/article/view/6404

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: hsa-miR-34a-5p enhances temozolomide anti-tumoral effects on glioblastoma ; volume:23 ; year:2024 ; EXCLI journal ; 23 (2024)

Contributors, Places and Time

• Creator: Abdoli Shadbad, Mahdi ; Baghbanzadeh, Amir ; Baradaran, Behzad

Further information

• DOI: 10.17179/excli2023-6404
• URN: urn:nbn:de:101:1-2406210313156.820327402658
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:49 AM CEST