Deciphering the regulatory landscape of fetal and adult [gamma delta] T‐cell development at single‐cell resolution

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Abstract: γδ T cells with distinct properties develop in the embryonic and adult thymus and have been identified as critical players in a broad range of infections, antitumor surveillance, autoimmune diseases, and tissue homeostasis. Despite their potential value for immunotherapy, differentiation of γδ T cells in the thymus is incompletely understood. Here, we establish a high‐resolution map of γδ T‐cell differentiation from the fetal and adult thymus using single‐cell RNA sequencing. We reveal novel sub‐types of immature and mature γδ T cells and identify an unpolarized thymic population which is expanded in the blood and lymph nodes. Our detailed comparative analysis reveals remarkable similarities between the gene networks active during fetal and adult γδ T‐cell differentiation. By performing a combined single‐cell analysis of Sox13, Maf, and Rorc knockout mice, we demonstrate sequential activation of these factors during IL‐17‐producing γδ T‐cell (γδT17) differentiation. These findings substantially expand our understanding of γδ T‐cell ontogeny in fetal and adult life. Our experimental and computational strategy provides a blueprint for comparing immune cell differentiation across developmental stages

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Notes: The EMBO journal. - 39, 13 (2020) , e104159, ISSN: 1460-2075

Event: Veröffentlichung

(where): Freiburg

(who): Universität

(when): 2020

Creator: Sagar, Stephen
Pokrovskii, Maria
Herman, Josip S.
Naik, Shruti
Sock, Elisabeth
Zeis, Patrice
Lausch, Ute
Wegner, Michael
Tanriver, Yakup
Littman, Dan R.
Grün, Dominic

DOI: 10.15252/embj.2019104159

URN: urn:nbn:de:bsz:25-freidok-1671658

Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 14.08.2025, 11:03 AM CEST

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Associated

Sagar, Stephen
Pokrovskii, Maria
Herman, Josip S.
Naik, Shruti
Sock, Elisabeth
Zeis, Patrice
Lausch, Ute
Wegner, Michael
Tanriver, Yakup
Littman, Dan R.
Grün, Dominic
Universität

Time of origin

2020

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Deciphering cancer cell state plasticity with single-cell genomics and artificial intelligence

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Deciphering the regulatory landscape of fetal and adult [gamma delta] T‐cell development at single‐cell resolution

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Other Objects (12)

Deciphering the regulatory landscape of fetal and adult γδ T‐cell development at single‐cell resolution

Deciphering and advancing CAR T-cell therapy with single-cell sequencing technologies

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Deciphering and advancing CAR T-cell therapy with single-cell sequencing technologies

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Single-cell T-cell receptor repertoire profiling in dogs

Deciphering cellular heterogeneity by single-cell transcriptome analysis

Deciphering primate retinal aging at single-cell resolution

Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq

Deciphering pediatric respiratory diseases using single-cell RNA sequencing

Single‐cell transcriptomics uncovers an instructive T‐cell receptor role in adult γδ T‐cell lineage commitment

Deciphering cancer cell state plasticity with single-cell genomics and artificial intelligence

Deciphering early human pancreas development at the single-cell level

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