Deciphering Cell–Cell Interactions with Integrative Single‐Cell Secretion Profiling
Abstract: Cell–cell interactions are the fundamental behaviors to regulate cellular activities. A comprehensive evaluation of intercellular interactions requires direct profiling of various signaling behaviors simultaneously at the single‐cell level, which remains lacking. Herein, an integrative single‐cell secretion analysis platform is presented to profile different secreted factors (four proteins, three extracellular vesicles (EV) phenotypes), spatial distances, and migration information (distances and direction) simultaneously from high‐throughput paired single cells using an antibody‐barcode microchip. Applying the platform to analyze the tumor–stromal and tumor–immune interactions with the human oral squamous cell carcinoma (OSCC) cell lines and primary OSCC cells reveals that the initial distances between cells would determine their migratory distances and direction to approach stable organization. The cell–cell in close proximity enhances protein secretions while attenuating EV secretions. Migration has a more profound correlation with protein secretions than EV secretions, in which absolute migration distance affects protein secretions significantly but not the direction. These findings highlight the significance of spatial organization in regulating cell signaling behaviors and demonstrate that the integrative single‐cell secretion profiling platform is well‐suited for a comprehensive dissection of intercellular communication and interactions, providing new avenues for understanding cell–cell interaction biology and how different signaling behaviors coordinate within the tumor microenvironment.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Deciphering Cell–Cell Interactions with Integrative Single‐Cell Secretion Profiling ; day:26 ; month:04 ; year:2023 ; extent:9
Advanced science ; (26.04.2023) (gesamt 9)
- Urheber
-
Li, Linmei
Su, Haoran
Ji, Yahui
Zhu, Fengjiao
Deng, Jiu
Bai, Xue
Li, Huibing
Liu, Xianming
Luo, Yong
Lin, Bingcheng
Liu, Tingjiao
Lu, Yao
- DOI
-
10.1002/advs.202301018
- URN
-
urn:nbn:de:101:1-2023042615330166041458
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
14.08.2025, 10:45 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Li, Linmei
- Su, Haoran
- Ji, Yahui
- Zhu, Fengjiao
- Deng, Jiu
- Bai, Xue
- Li, Huibing
- Liu, Xianming
- Luo, Yong
- Lin, Bingcheng
- Liu, Tingjiao
- Lu, Yao
Ähnliche Objekte (12)
Test unterschiedlicher FE-Programme für die praktische Ausbildung
Gemeinsames Lernen von Kommunikationsprotokollen durch elektronisch unterstützes Rollenspiel
A Cross-Linguistic Exploration of Context Updating Mechanisms
Applications of MXene‐Based Single‐Atom Catalysts
Is There Cross-Cycle Adjustment in China’s Monetary Policy?
FOXM1 promote the growth and metastasis of uveal melanoma cells by regulating CDK2 expression
Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering
Cell-Cell Channels
Approach and assessment of automated stereotactic radiotherapy planning for early stage non-small-cell lung cancer
The Influence of Parent- and Adult Child-level Factors on Intergenerational Relationship Quality: A Study of Chinese Families with Multiple Children in Hong Kong
A pseudo-sluicing analysis of reduced embedded questions in Chakhar Mongolian
Influence of intergenerational relationships on depressive symptoms in ageing Chinese adults in Hong Kong: Mediating effects of sense of loneliness
Test unterschiedlicher FE-Programme für die praktische Ausbildung
Gemeinsames Lernen von Kommunikationsprotokollen durch elektronisch unterstützes Rollenspiel
A Cross-Linguistic Exploration of Context Updating Mechanisms
Applications of MXene‐Based Single‐Atom Catalysts
Is There Cross-Cycle Adjustment in China’s Monetary Policy?
FOXM1 promote the growth and metastasis of uveal melanoma cells by regulating CDK2 expression
Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering
Cell-Cell Channels
Approach and assessment of automated stereotactic radiotherapy planning for early stage non-small-cell lung cancer
The Influence of Parent- and Adult Child-level Factors on Intergenerational Relationship Quality: A Study of Chinese Families with Multiple Children in Hong Kong
A pseudo-sluicing analysis of reduced embedded questions in Chakhar Mongolian
Influence of intergenerational relationships on depressive symptoms in ageing Chinese adults in Hong Kong: Mediating effects of sense of loneliness
Test unterschiedlicher FE-Programme für die praktische Ausbildung
Gemeinsames Lernen von Kommunikationsprotokollen durch elektronisch unterstützes Rollenspiel
A Cross-Linguistic Exploration of Context Updating Mechanisms
Applications of MXene‐Based Single‐Atom Catalysts
Is There Cross-Cycle Adjustment in China’s Monetary Policy?
FOXM1 promote the growth and metastasis of uveal melanoma cells by regulating CDK2 expression
Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering
Cell-Cell Channels
Approach and assessment of automated stereotactic radiotherapy planning for early stage non-small-cell lung cancer
The Influence of Parent- and Adult Child-level Factors on Intergenerational Relationship Quality: A Study of Chinese Families with Multiple Children in Hong Kong
A pseudo-sluicing analysis of reduced embedded questions in Chakhar Mongolian