Extrusion-based 3D bioprinting of gradients of stiffness, cell density, and immobilized peptide using thermogelling hydrogels
Abstract: To study biological processes in vitro, biomaterials-based engineering solutions to reproduce the gradients observed in tissues are necessary. We present a platform for the 3D bioprinting of functionally graded biomaterials based on carboxylated agarose, a bioink amendable by extrusion bioprinting. Using this bioink, objects with a gradient of stiffness and gradient of cell concentration were printed. Functionalization of carboxylated agarose with maleimide moieties that react in minutes with a cysteine-terminated cell-adhesion peptide allowed us to print objects with a gradient of an immobilized peptide. This approach paves the way toward the development of tissue mimics with gradients
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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ACS biomaterials science & engineering. - 7, 6 (2021) , 2192-2197, ISSN: 2373-9878
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2024
- Urheber
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Kuzucu, Merve
Vera, Grace
Beaumont, Marco
Fischer, Sascha
Wei, Pan
Shastri, V. Prasad
Forget, Aurelien
- DOI
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10.1021/acsbiomaterials.1c00183
- URN
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urn:nbn:de:bsz:25-freidok-2477566
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:53 MEZ
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Beteiligte
- Kuzucu, Merve
- Vera, Grace
- Beaumont, Marco
- Fischer, Sascha
- Wei, Pan
- Shastri, V. Prasad
- Forget, Aurelien
- Universität
Entstanden
- 2024
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