Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells

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Abstract: Mechanical stimulation plays in an important role in regulating stem cell differentiation and their release of extracellular vesicles (EVs). In this study, effects of low magnitude hydrostatic pressure (HP) on the chondrogenic differentiation and microvesicle release from human embryonic stem cells (hESCs) and human bone marrow stem cells (hBMSCs) are examined. hESCs were differentiated into chondroprogenitors and then embedded in fibrin gels and subjected to HP (270 kPa, 1 Hz, 5 days per week). hBMSC pellets were differentiated in chondrogenic media and subjected to the same regime. HP significantly enhanced ACAN expression in hESCs. It also led to a significant increase in DNA content, sGAG content and total sGAG/DNA level in hBMSCs. Furthermore, HP significantly increased microvesicle protein content released from both cell types. These results highlight the benefit of HP bioreactor in promoting chondrogenesis and EV production for cartilage tissue engineering.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells ; day:17 ; month:01 ; year:2022 ; extent:11
Biotechnology journal ; (17.01.2022) (gesamt 11)

Urheber
Luo, Lu
Foster, Nicola C.
Man, Kenny L.
Brunet, Mathieu
Hoey, David A.
Cox, Sophie C.
Kimber, Susan J.
El Haj, Alicia J.

DOI
10.1002/biot.202100401
URN
urn:nbn:de:101:1-2022011714131747679504
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:21 MESZ

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Beteiligte

  • Luo, Lu
  • Foster, Nicola C.
  • Man, Kenny L.
  • Brunet, Mathieu
  • Hoey, David A.
  • Cox, Sophie C.
  • Kimber, Susan J.
  • El Haj, Alicia J.

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