A novel micropeptide, Slitharin, exerts cardioprotective effects in myocardial infarction
Purpose: Micropeptides are an emerging class of proteins that play critical roles in cell signaling. Here, we describe the discovery of a novel micropeptide, dubbed slitharin (Slt), in conditioned media from Cardiosphere‐derived cells (CDCs), a therapeutic cardiac stromal cell type. Experimental design: We performed mass spectrometry of peptide‐enriched fractions from the conditioned media of CDCs and a therapeutically inert cell type (human dermal fibrobasts). We then evaluated the therapeutic capacity of the candidate peptide using an in vitro model of cardiomyocyte injury and a rat model of myocardial infarction. Results: We identified a novel 24‐amino acid micropeptide (dubbed Slitharin [Slt]) with a non‐canonical leucine start codon, arising from long intergenic non‐coding (LINC) RNA 2099. Neonatal rat ventricular myocytes (NRVMs) exposed to Slt were protected from hypoxic injury in vitro compared to a vehicle or scrambled control. Transcriptomic analysis of cardiomyocytes exposed to Slt reveals cytoprotective capacity, putatively through regulation of stress‐induced MAPK‐ERK. Slt also exerted cardioprotective effects in rats with myocardial infarction as shown by reduced infarct size 48 h post‐injury. Conclusions and clinical relavance: Thus, Slt is a non‐coding RNA‐derived micropeptide, identified in the extracellular space, with a potential cardioprotective function.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
A novel micropeptide, Slitharin, exerts cardioprotective effects in myocardial infarction ; day:05 ; month:03 ; year:2024 ; extent:9
Proteomics / Clinical applications. Clinical applications ; (05.03.2024) (gesamt 9)
- Creator
-
Ibrahim, Ahmed G. E.
Ciullo, Alessandra
Yamaguchi, Shukuro
Li, Chang
Antes, Travis
Jones, Xaviar
Li, Liang
Murali, Ramachandran
Maslennikov, Innokentiy
Sundararaman, Niveda
Soetkamp, Daniel
Cingolani, Eugenio
Van Eyk, Jennifer
Marbán, Eduardo
- DOI
-
10.1002/prca.202300128
- URN
-
urn:nbn:de:101:1-2024030614342196696737
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
14.08.2025, 11:01 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Ibrahim, Ahmed G. E.
- Ciullo, Alessandra
- Yamaguchi, Shukuro
- Li, Chang
- Antes, Travis
- Jones, Xaviar
- Li, Liang
- Murali, Ramachandran
- Maslennikov, Innokentiy
- Sundararaman, Niveda
- Soetkamp, Daniel
- Cingolani, Eugenio
- Van Eyk, Jennifer
- Marbán, Eduardo
Other Objects (12)
Isolierung und Charakterisierung adulter mesenchymaler Stroma-/Stammzellen aus humanem Thymusgewebe und deren potentielle therapeutische Möglichkeiten
Book review on Workgroups e-assessment: planning, implementing and analyzing frameworks
Development of an automated milling system for the decontamination of the wall surface in a nuclear power plant
Sampling investigation and statistical analysis for mental problems of freshmen in Shandong Province
Internet use and frailty in middle-aged and older adults: findings from developed and developing countries
Competing endogenous RNA network analysis explores the key lncRNAs, miRNAs, and mRNAs in type 1 diabetes
The benefits of swimming together
Characterizations of the dilation of frame generator dual pairs for group
Hysterothylacium gibsoni sp. nov. and H. tetrapteri (Bruce et Cannon, 1989) (Nematoda: Ascaridida) from the Chinese marine fishes
A new species of genus Hysterothylacium Ward et Magath, 1917 (Nematoda, Anisakidae) from Liparis tanakae (Scorpaeniformes, Liparidae) from the Yellow Sea, China
Roles of extracellular microRNAs in central nervous system
Graded energy band engineering for efficient perovskite solar cells
Isolierung und Charakterisierung adulter mesenchymaler Stroma-/Stammzellen aus humanem Thymusgewebe und deren potentielle therapeutische Möglichkeiten
Book review on Workgroups e-assessment: planning, implementing and analyzing frameworks
Development of an automated milling system for the decontamination of the wall surface in a nuclear power plant
Sampling investigation and statistical analysis for mental problems of freshmen in Shandong Province
Internet use and frailty in middle-aged and older adults: findings from developed and developing countries
Competing endogenous RNA network analysis explores the key lncRNAs, miRNAs, and mRNAs in type 1 diabetes
The benefits of swimming together
Characterizations of the dilation of frame generator dual pairs for group
Hysterothylacium gibsoni sp. nov. and H. tetrapteri (Bruce et Cannon, 1989) (Nematoda: Ascaridida) from the Chinese marine fishes
A new species of genus Hysterothylacium Ward et Magath, 1917 (Nematoda, Anisakidae) from Liparis tanakae (Scorpaeniformes, Liparidae) from the Yellow Sea, China
Roles of extracellular microRNAs in central nervous system
Graded energy band engineering for efficient perovskite solar cells
Isolierung und Charakterisierung adulter mesenchymaler Stroma-/Stammzellen aus humanem Thymusgewebe und deren potentielle therapeutische Möglichkeiten
Book review on Workgroups e-assessment: planning, implementing and analyzing frameworks
Development of an automated milling system for the decontamination of the wall surface in a nuclear power plant
Sampling investigation and statistical analysis for mental problems of freshmen in Shandong Province
Internet use and frailty in middle-aged and older adults: findings from developed and developing countries
Competing endogenous RNA network analysis explores the key lncRNAs, miRNAs, and mRNAs in type 1 diabetes
The benefits of swimming together
Characterizations of the dilation of frame generator dual pairs for group
Hysterothylacium gibsoni sp. nov. and H. tetrapteri (Bruce et Cannon, 1989) (Nematoda: Ascaridida) from the Chinese marine fishes
A new species of genus Hysterothylacium Ward et Magath, 1917 (Nematoda, Anisakidae) from Liparis tanakae (Scorpaeniformes, Liparidae) from the Yellow Sea, China
Roles of extracellular microRNAs in central nervous system