A drop-on-demand bioprinting approach to spatial arrangement of multiple cell types and monitoring their cell–cell interactions towards vascularization based on endothelial cells and mesenchymal stem cells
Abstract: Spheroids, organoids, or cell-laden droplets are often used as building blocks for bioprinting, but so far little is known about the spatio-temporal cellular interactions subsequent to printing. We used a drop-on-demand bioprinting approach to study the biological interactions of such building blocks in dimensions of micrometers. Highly-density droplets (approximately 700 cells in 10 nL) of multiple cell types were patterned in a 3D hydrogel matrix with a precision of up to 70 μm. The patterns were used to investigate interactions of endothelial cells (HUVECs) and adipose-derived mesenchymal stem cells (ASCs), which are related to vascularization. We demonstrated that a gap of 200 μm between HUVEC and ASC aggregates led to decreased sprouting of HUVECs towards ASCs and increased growth from ASCs towards HUVECs. For mixed aggregates containing both cell types, cellular interconnections of ASCs with lengths of up to approximately 800 µm and inhibition of HUVEC sprouting were observed. When ASCs were differentiated into smooth muscle cells (dASCs), separate HUVEC aggregates displayed decreased sprouting towards dASCs, whereas no cellular interconnections nor inhibition of HUVEC sprouting were detected for mixed dASCs/HUVEC aggregates. These findings demonstrate that our approach could be applied to investigate cell–cell interactions of different cell types in 3D co-cultures
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Cells. - 12, 4 (2023) , 646, ISSN: 2073-4409
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
-
Universität
- (when)
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2023
- Creator
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Weygant, Joshua
Koch, Fritz
Adam, Katrin
Tröndle, Kevin
Zengerle, Roland
Finkenzeller, Günter
Kartmann, Sabrina
Koltay, Peter
Zimmermann, Stefan
- DOI
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10.3390/cells12040646
- URN
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urn:nbn:de:bsz:25-freidok-2336512
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:44 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Weygant, Joshua
- Koch, Fritz
- Adam, Katrin
- Tröndle, Kevin
- Zengerle, Roland
- Finkenzeller, Günter
- Kartmann, Sabrina
- Koltay, Peter
- Zimmermann, Stefan
- Albert-Ludwigs-Universität Freiburg. Professur für Anwendungsentwicklung
- Universität
Time of origin
- 2023
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