Accessible and reproducible mass spectrometry imaging data analysis in Galaxy

Abstract: Background
Mass spectrometry imaging is increasingly used in biological and translational research because it has the ability to determine the spatial distribution of hundreds of analytes in a sample. Being at the interface of proteomics/metabolomics and imaging, the acquired datasets are large and complex and often analyzed with proprietary software or in-house scripts, which hinders reproducibility. Open source software solutions that enable reproducible data analysis often require programming skills and are therefore not accessible to many mass spectrometry imaging (MSI) researchers.

Findings
We have integrated 18 dedicated mass spectrometry imaging tools into the Galaxy framework to allow accessible, reproducible, and transparent data analysis. Our tools are based on Cardinal, MALDIquant, and scikit-image and enable all major MSI analysis steps such as quality control, visualization, preprocessing, statistical analysis, and image co-registration. Furthermore, we created hands-on training material for use cases in proteomics and metabolomics. To demonstrate the utility of our tools, we re-analyzed a publicly available N-linked glycan imaging dataset. By providing the entire analysis history online, we highlight how the Galaxy framework fosters transparent and reproducible research.

Conclusion
The Galaxy framework has emerged as a powerful analysis platform for the analysis of MSI data with ease of use and access, together with high levels of reproducibility and transparency

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: GigaScience. - 8, 12 (2019) , giz143, ISSN: 2047-217X

Klassifikation und Themen

• Schlagwort: Massenspektrometrie ; MALDI-MS ; Proteomanalyse ; Metabolomik

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2020
• Urheber: Föll, Melanie Christine ; Moritz, Lennart ; Wollmann, Thomas ; Stillger, Maren Nicole ; Vockert, Niklas ; Werner, Martin ; Bronsert, Peter ; Rohr, Karl ; Grüning, Björn ; Schilling, Oliver

Weitere Informationen

• DOI: 10.1093/gigascience/giz143
• URN: urn:nbn:de:bsz:25-freidok-1517194
• Rechteinformation: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 25.03.20242024, 13:46 MEZ