Directly reprogrammed neural precursors from patient derived fibroblasts
Abstract: Cellular therapy experienced an important shift with the discovery of pluripotent stem cells and with the reinvention of these through the forced expression of four pluripotency related transcription factors (Takahashi and Yamanaka, 2006); describing a protocol that drove a somatic cell backwards into an induced pluripotent state (induced Pluripotent Stem cells, iPS cells).
Nevertheless, the process remained inefficient and involved practical queries as the risk of mutations, re-activation of the vectors, and use of potential oncogenes, without forgetting the fact that a pluripotent cell itself may serve as a potential source for tumors.
To overcome those difficulties, different approaches were described: like the substitution of the original transcription factors with small molecules or sorting pluripotency by direct reprogramming into another mature phenotype.
Addition of small molecules not only substituted the transcription factors but also improved the efficiency of the whole process, while simultaneously, new approaches tried to sort the risks of integration and reactivation of the previously described vectors (lentivirus, adenovirus, proteins, etc.).
The present work describes our pathway towards a patient’s derived cellular model of neural progenitors based on the work of Takahashi and Yamanaka and on a transfection insertion free method (Okita et. Al. 2011), giving birth to an induced Neural Precursor cell line (iNSC’s), a tool in order to understand a subject’s specific disease from an in vitro and an in vivo perspectives
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Universität Freiburg, Dissertation, 2018
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2019
- Creator
- Contributor
- DOI
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10.6094/UNIFR/17531
- URN
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urn:nbn:de:bsz:25-freidok-175316
- Rights
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:48 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Azmitia, Luis
- Nikkhah, Guido
- Schültke, Elisabeth
- Albert-Ludwigs-Universität Freiburg. Medizinische Fakultät
- Universität
Time of origin
- 2019
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