The NSL complex is required for piRNA production from telomeric clusters
Abstract: The NSL complex is a transcriptional activator. Germline-specific knockdown of NSL complex subunits NSL1, NSL2, and NSL3 results in reduced piRNA production from a subset of bidirectional piRNA clusters, accompanied by widespread transposon derepression. The piRNAs most transcriptionally affected by NSL2 and NSL1 RNAi map to telomeric piRNA clusters. At the chromatin level, these piRNA clusters also show decreased levels of H3K9me3, HP1a, and Rhino after NSL2 depletion. Using NSL2 ChIP-seq in ovaries, we found that this protein specifically binds promoters of telomeric transposons HeT-A, TAHRE, and TART. Germline-specific depletion of NSL2 also led to a reduction in nuclear Piwi in nurse cells. Our findings thereby support a role for the NSL complex in promoting the transcription of piRNA precursors from telomeric piRNA clusters and in regulating Piwi levels in the Drosophila female germline
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Life science alliance. - 6, 9 (2023) , e202302194, ISSN: 2575-1077
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2024
- Creator
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Iyer, Shantanu S.
Sun, Yidan
Seyfferth, Janine
Manjunath, Vinitha
Samata, Maria
Alexiadis, Anastasios
Kulkarni, Tanvi
Gutierrez, Noel
Georgiev, Plamen
Shvedunova, Maria
Akhtar, Asifa
- DOI
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10.26508/lsa.202302194
- URN
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urn:nbn:de:bsz:25-freidok-2540324
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:54 PM CET
Data provider
This object is provided by:
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Iyer, Shantanu S.
- Sun, Yidan
- Seyfferth, Janine
- Manjunath, Vinitha
- Samata, Maria
- Alexiadis, Anastasios
- Kulkarni, Tanvi
- Gutierrez, Noel
- Georgiev, Plamen
- Shvedunova, Maria
- Akhtar, Asifa
- Universität
Time of origin
- 2024
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